1H NMR metabonomic study of rat response to tri-phenyl phosphate and tri-butyl phosphate exposure

Todd M. Alam, M. Kathleen Alam, Muniasamy Neerathilingam, David E. Volk, S. Sarkar, Ghulam Ansari, Bruce A. Luxon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The industrial application of organophosphates provides the opportunity for environmental exposure. While the toxicity of organophosphate compounds has been the target of significant work, studies directed towards the identification of metabolite markers to assess phosphate exposure are more limited. In this study the urine metabolite profiles for rats following single dose exposure to either tributyl phosphate (TBP, 15 mg/kg body weight) or triphenyl phosphate (TPP, 2 and 20 mg/kg body weight) were characterized using proton nuclear magnetic resonance (1H NMR) and orthogonal-partial least squares discriminate analysis (O-PLSDA). Using the developed O-PLSDA models it was possible to clearly identify TBP or TPP exposed animals. The performance of these models was validated using cross validation and permutation testing. Utilizing the variable importance in projection (VIP) coefficients from the O-PLSDA the metabolites that were most responsible for the classification of TBP or TPP exposure were determined. This initial study demonstrates the potential for NMR metabonomic studies for the identification and separation of environmental exposure to organophosphates.

Original languageEnglish (US)
Pages (from-to)386-394
Number of pages9
JournalMetabolomics
Volume6
Issue number3
DOIs
StatePublished - 2010

Fingerprint

Metabolomics
Organophosphates
Rats
Metabolites
Least-Squares Analysis
Phosphates
Nuclear magnetic resonance
Environmental Exposure
Body Weight
Industrial applications
Toxicity
Protons
Animals
Magnetic Resonance Spectroscopy
Urine
tributyl phosphate
Proton Magnetic Resonance Spectroscopy
Testing

Keywords

  • Chemometrics
  • Discriminate analysis
  • Metabolomics
  • Metabonomics
  • Nuclear magnetic resonance (NMR)
  • Organophosphate toxicology
  • Urine metabolites

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Alam, T. M., Alam, M. K., Neerathilingam, M., Volk, D. E., Sarkar, S., Ansari, G., & Luxon, B. A. (2010). 1H NMR metabonomic study of rat response to tri-phenyl phosphate and tri-butyl phosphate exposure. Metabolomics, 6(3), 386-394. https://doi.org/10.1007/s11306-010-0205-z

1H NMR metabonomic study of rat response to tri-phenyl phosphate and tri-butyl phosphate exposure. / Alam, Todd M.; Alam, M. Kathleen; Neerathilingam, Muniasamy; Volk, David E.; Sarkar, S.; Ansari, Ghulam; Luxon, Bruce A.

In: Metabolomics, Vol. 6, No. 3, 2010, p. 386-394.

Research output: Contribution to journalArticle

Alam, TM, Alam, MK, Neerathilingam, M, Volk, DE, Sarkar, S, Ansari, G & Luxon, BA 2010, '1H NMR metabonomic study of rat response to tri-phenyl phosphate and tri-butyl phosphate exposure', Metabolomics, vol. 6, no. 3, pp. 386-394. https://doi.org/10.1007/s11306-010-0205-z
Alam, Todd M. ; Alam, M. Kathleen ; Neerathilingam, Muniasamy ; Volk, David E. ; Sarkar, S. ; Ansari, Ghulam ; Luxon, Bruce A. / 1H NMR metabonomic study of rat response to tri-phenyl phosphate and tri-butyl phosphate exposure. In: Metabolomics. 2010 ; Vol. 6, No. 3. pp. 386-394.
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