1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz

Maruthi Kashyap; Zeenia Jagga; Bhaba Krishna Das; Arulandu Arockiasamy; Neel Sarovar Bhavesh

doi:10.1007/s12104-011-9331-4

¹H, ¹³C and ¹⁵N NMR assignments of inactive form of P1 endolysin Lyz

Maruthi Kashyap
, Zeenia Jagga
, Bhaba Krishna Das
, Arulandu Arockiasamy
, Neel Sarovar Bhavesh

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific ¹H, ¹³C and ¹⁵N resonance assignments for secreted inactive form of Lyz at pH 4.5.

Original language	English (US)
Pages (from-to)	87-89
Number of pages	3
Journal	Biomolecular NMR Assignments
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s12104-011-9331-4
State	Published - Apr 2012
Externally published	Yes

Keywords

Cell lysis
Endolysin
Phage P1

ASJC Scopus subject areas

Structural Biology
Biochemistry

Access to Document

10.1007/s12104-011-9331-4

Cite this

@article{6f056216ccc04d4fbacdbf019a802b9f,

title = "1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz",

abstract = "Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.",

keywords = "Cell lysis, Endolysin, Phage P1",

author = "Maruthi Kashyap and Zeenia Jagga and Das, \{Bhaba Krishna\} and Arulandu Arockiasamy and Bhavesh, \{Neel Sarovar\}",

year = "2012",

month = apr,

doi = "10.1007/s12104-011-9331-4",

language = "English (US)",

volume = "6",

pages = "87--89",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

TY - JOUR

T1 - 1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz

AU - Kashyap, Maruthi

AU - Jagga, Zeenia

AU - Das, Bhaba Krishna

AU - Arockiasamy, Arulandu

AU - Bhavesh, Neel Sarovar

PY - 2012/4

Y1 - 2012/4

N2 - Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.

AB - Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.

KW - Cell lysis

KW - Endolysin

KW - Phage P1

UR - https://www.scopus.com/pages/publications/84858182607

UR - https://www.scopus.com/pages/publications/84858182607#tab=citedBy

U2 - 10.1007/s12104-011-9331-4

DO - 10.1007/s12104-011-9331-4

M3 - Article

C2 - 21822941

AN - SCOPUS:84858182607

SN - 1874-2718

VL - 6

SP - 87

EP - 89

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 1

ER -