Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR

Tariq A. Madani, El Tayeb M E Abuelzein, Esam I. Azhar, Moujahed Kao, Hussein M S Al-Bar, Huda Abu-Araki, Thomas Ksiazek

Research output: Contribution to journalArticle

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Abstract

RT-PCR to detect Alkhumra virus (ALKV) RNA in plasma or serum has been the standard practice to confirm this infection in the first seven days of illness. In this study, RT-PCR detection of viral RNA from the plasma, serum, and buffy coat (BC) was compared to virus isolation. Plasma, serum, and BC were obtained from seven patients with clinically suspected ALKV infection in Najran, Saudi Arabia. Baby hamster kidney (BHK-21) and rhesus monkey kidney (LLC-MK2) cell culture monolayers were used for virus isolation. Real-time RT-PCR was used to confirm ALKV infection and to detect viral RNA directly from plasma, serum, and BC. ALKV was isolated from five of the seven patients. The virus was isolated from all three specimen types (plasma, serum, and BC) of the five confirmed patients. ALKV RNA was detected directly by RT-PCR in BC in all five (100%) culture-positive patients and in plasma or serum in only four (80%) of the five patients. Three of the five patients for whom ALKV RNA was detected in BC also had detectable viral RNA in plasma and serum. In the remaining two patients with detectable ALKV RNA in the BC, the plasma was positive but the serum was negative in one patient, whereas the serum was positive and the plasma was negative in the other patient. The use of real-time RT-PCR to detect ALKV RNA in the BC was superior to using plasma and serum and equivalent to virus isolation.

Original languageEnglish (US)
Pages (from-to)819-823
Number of pages5
JournalArchives of Virology
Volume157
Issue number5
DOIs
StatePublished - May 2012

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RNA Viruses
Real-Time Polymerase Chain Reaction
Serum
Viruses
Viral RNA
Virus Diseases
Polymerase Chain Reaction
Kidney
Sick Leave
Saudi Arabia
Macaca mulatta
Cricetinae
Cell Culture Techniques

ASJC Scopus subject areas

  • Virology

Cite this

Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR. / Madani, Tariq A.; Abuelzein, El Tayeb M E; Azhar, Esam I.; Kao, Moujahed; Al-Bar, Hussein M S; Abu-Araki, Huda; Ksiazek, Thomas.

In: Archives of Virology, Vol. 157, No. 5, 05.2012, p. 819-823.

Research output: Contribution to journalArticle

Madani, Tariq A. ; Abuelzein, El Tayeb M E ; Azhar, Esam I. ; Kao, Moujahed ; Al-Bar, Hussein M S ; Abu-Araki, Huda ; Ksiazek, Thomas. / Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR. In: Archives of Virology. 2012 ; Vol. 157, No. 5. pp. 819-823.
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