Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock

Z. F. Xia, M. Hollyoak, R. E. Barrow, F. He, M. J. Muller, David Herndon

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Delayed fluid resuscitation during burn shock is thought to compromise the integrity of gut mucosa and allow enteric bacteria to cross the luminal wall and infect other sterile organ systems. Superoxide dismutase, a free-oxygen radical scavenger; leupeptin, a protease inhibitor; and verapamil, a calcium channel blocker, were studied to evaluate their efficacy in maintaining cellular integrity in the gut of thermally burned rats whose fluid resuscitation had been delayed. Fifty male rats weighing 280 to 320 gm were given a full-thickness scald burn covering 50% total body surface area. Ten received early fluid resuscitation beginning half an hour after burn, and 40 received fluid resuscitation delayed by 6 hours. Those receiving delayed resuscitation were given superoxide dismutase (n = 10), leupeptin (n = 10), verapamil (n = 10), or a placebo of normal saline solution (n = 10) at the time of fluid resuscitation. Ileal mucosa samples were harvested, and adenosine triphosphate, diphosphate, and monophosphate were measured. Adenosine triphosphate, total nucleotides, and energy charge potential were significantly lower in the placebo group without therapy compared with those of the early resuscitation group. Superoxide dismutase and leupeptin therapy prevented this drop in cellular energy. Total water content was significantly increased in the placebo group compared with that of the early resuscitation group; superoxide dismutase was able to prevent this increase. Data indicate that intestinal reperfusion injury in burned rats can be effectively modulated with superoxide dismutase or leupeptin therapy.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalJournal of Burn Care and Rehabilitation
Volume16
Issue number2 I
DOIs
StatePublished - 1995

Fingerprint

Reperfusion Injury
Resuscitation
Superoxide Dismutase
Small Intestine
Shock
Placebos
Verapamil
Mucous Membrane
Free Radical Scavengers
leupeptin
Body Surface Area
Calcium Channel Blockers
Enterobacteriaceae
Group Psychotherapy
Protease Inhibitors
Sodium Chloride
Adenosine Diphosphate
Reactive Oxygen Species
Nucleotides
Adenosine Triphosphate

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock. / Xia, Z. F.; Hollyoak, M.; Barrow, R. E.; He, F.; Muller, M. J.; Herndon, David.

In: Journal of Burn Care and Rehabilitation, Vol. 16, No. 2 I, 1995, p. 111-117.

Research output: Contribution to journalArticle

Xia, Z. F. ; Hollyoak, M. ; Barrow, R. E. ; He, F. ; Muller, M. J. ; Herndon, David. / Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock. In: Journal of Burn Care and Rehabilitation. 1995 ; Vol. 16, No. 2 I. pp. 111-117.
@article{0698c2fe8dda4c619d7323a68df04cbe,
title = "Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock",
abstract = "Delayed fluid resuscitation during burn shock is thought to compromise the integrity of gut mucosa and allow enteric bacteria to cross the luminal wall and infect other sterile organ systems. Superoxide dismutase, a free-oxygen radical scavenger; leupeptin, a protease inhibitor; and verapamil, a calcium channel blocker, were studied to evaluate their efficacy in maintaining cellular integrity in the gut of thermally burned rats whose fluid resuscitation had been delayed. Fifty male rats weighing 280 to 320 gm were given a full-thickness scald burn covering 50{\%} total body surface area. Ten received early fluid resuscitation beginning half an hour after burn, and 40 received fluid resuscitation delayed by 6 hours. Those receiving delayed resuscitation were given superoxide dismutase (n = 10), leupeptin (n = 10), verapamil (n = 10), or a placebo of normal saline solution (n = 10) at the time of fluid resuscitation. Ileal mucosa samples were harvested, and adenosine triphosphate, diphosphate, and monophosphate were measured. Adenosine triphosphate, total nucleotides, and energy charge potential were significantly lower in the placebo group without therapy compared with those of the early resuscitation group. Superoxide dismutase and leupeptin therapy prevented this drop in cellular energy. Total water content was significantly increased in the placebo group compared with that of the early resuscitation group; superoxide dismutase was able to prevent this increase. Data indicate that intestinal reperfusion injury in burned rats can be effectively modulated with superoxide dismutase or leupeptin therapy.",
author = "Xia, {Z. F.} and M. Hollyoak and Barrow, {R. E.} and F. He and Muller, {M. J.} and David Herndon",
year = "1995",
doi = "10.1097/00004630-199503000-00004",
language = "English (US)",
volume = "16",
pages = "111--117",
journal = "Journal of Burn Care and Research",
issn = "1559-047X",
publisher = "Lippincott Williams and Wilkins",
number = "2 I",

}

TY - JOUR

T1 - Superoxide dismutase and leupeptin prevent delayed reperfusion injury in the rat small intestine during burn shock

AU - Xia, Z. F.

AU - Hollyoak, M.

AU - Barrow, R. E.

AU - He, F.

AU - Muller, M. J.

AU - Herndon, David

PY - 1995

Y1 - 1995

N2 - Delayed fluid resuscitation during burn shock is thought to compromise the integrity of gut mucosa and allow enteric bacteria to cross the luminal wall and infect other sterile organ systems. Superoxide dismutase, a free-oxygen radical scavenger; leupeptin, a protease inhibitor; and verapamil, a calcium channel blocker, were studied to evaluate their efficacy in maintaining cellular integrity in the gut of thermally burned rats whose fluid resuscitation had been delayed. Fifty male rats weighing 280 to 320 gm were given a full-thickness scald burn covering 50% total body surface area. Ten received early fluid resuscitation beginning half an hour after burn, and 40 received fluid resuscitation delayed by 6 hours. Those receiving delayed resuscitation were given superoxide dismutase (n = 10), leupeptin (n = 10), verapamil (n = 10), or a placebo of normal saline solution (n = 10) at the time of fluid resuscitation. Ileal mucosa samples were harvested, and adenosine triphosphate, diphosphate, and monophosphate were measured. Adenosine triphosphate, total nucleotides, and energy charge potential were significantly lower in the placebo group without therapy compared with those of the early resuscitation group. Superoxide dismutase and leupeptin therapy prevented this drop in cellular energy. Total water content was significantly increased in the placebo group compared with that of the early resuscitation group; superoxide dismutase was able to prevent this increase. Data indicate that intestinal reperfusion injury in burned rats can be effectively modulated with superoxide dismutase or leupeptin therapy.

AB - Delayed fluid resuscitation during burn shock is thought to compromise the integrity of gut mucosa and allow enteric bacteria to cross the luminal wall and infect other sterile organ systems. Superoxide dismutase, a free-oxygen radical scavenger; leupeptin, a protease inhibitor; and verapamil, a calcium channel blocker, were studied to evaluate their efficacy in maintaining cellular integrity in the gut of thermally burned rats whose fluid resuscitation had been delayed. Fifty male rats weighing 280 to 320 gm were given a full-thickness scald burn covering 50% total body surface area. Ten received early fluid resuscitation beginning half an hour after burn, and 40 received fluid resuscitation delayed by 6 hours. Those receiving delayed resuscitation were given superoxide dismutase (n = 10), leupeptin (n = 10), verapamil (n = 10), or a placebo of normal saline solution (n = 10) at the time of fluid resuscitation. Ileal mucosa samples were harvested, and adenosine triphosphate, diphosphate, and monophosphate were measured. Adenosine triphosphate, total nucleotides, and energy charge potential were significantly lower in the placebo group without therapy compared with those of the early resuscitation group. Superoxide dismutase and leupeptin therapy prevented this drop in cellular energy. Total water content was significantly increased in the placebo group compared with that of the early resuscitation group; superoxide dismutase was able to prevent this increase. Data indicate that intestinal reperfusion injury in burned rats can be effectively modulated with superoxide dismutase or leupeptin therapy.

UR - http://www.scopus.com/inward/record.url?scp=0028953936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028953936&partnerID=8YFLogxK

U2 - 10.1097/00004630-199503000-00004

DO - 10.1097/00004630-199503000-00004

M3 - Article

VL - 16

SP - 111

EP - 117

JO - Journal of Burn Care and Research

JF - Journal of Burn Care and Research

SN - 1559-047X

IS - 2 I

ER -