Superoxide generation and reversal of acetylcholine-induced cerebral arteriolar dilation after acute hypertension

E. P. Wei, H. A. Kontos, C. W. Christman, Douglas Dewitt, J. T. Povlishock

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

The appearance of superoxide anion radical in cerebral extracellular space during and after acute hypertension induced by intravenous norepinephrine was investigated in anesthetized cats equipped with cranial windows. Superoxide was detected by demonstrating the presence of superoxide dismutase-inhibitable reduction of nitroblue tetrazolium. The superoxide dismutase-inhibitable rate of reduction of nitroblue tetrazolium was 4.1 ± 1.61 nM/min per cm2 during hypertension and 4.55 ± 0.62 nM/min per cm2 one hour after hypertension had subsided. During norepinephrine administration in the absence of hypertension, the superoxide dismutase-inhibitable rate of reduction of nitroblue tetrazolium was 0.44 ± 0.17 nM/min per cm2. The reduction of nitroblue tetrazolium during hypertension was also inhibited by prior treatment of the brain surface with phenylglyoxal at pH 10, to induce irreversible inhibition of the anion channel. The results show that acute hypertension is associated with the generation of superoxide which enters the extracellular space of the brain via the anion channel. Following hypertension, the sustained vasodilation caused by acute hypertension was inhibited significantly by topical application of superoxide dismutase and catalase, showing that it was due in part to superoxide and other radicals derived from it. The vasodilator response of cerebral arterioles to topical acetylcholine was converted to vasoconstriction following acute hypertension, and restored to vasodilation following topical application of superoxide dismutase and catalase. The results show that superoxide and other radicals generated after acute hypertension interfere with acetylcholine-induced endothelium-dependent vasodilation, probably because they destroy the endothelium-derived relaxant factor.

Original languageEnglish (US)
Pages (from-to)781-787
Number of pages7
JournalCirculation Research
Volume57
Issue number5
StatePublished - 1985
Externally publishedYes

Fingerprint

Superoxides
Acetylcholine
Dilatation
Hypertension
Nitroblue Tetrazolium
Superoxide Dismutase
Vasodilation
Extracellular Space
Catalase
Anions
Norepinephrine
Phenylglyoxal
Endothelium-Dependent Relaxing Factors
Brain
Arterioles
Vasoconstriction
Vasodilator Agents
Endothelium
Cats

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Superoxide generation and reversal of acetylcholine-induced cerebral arteriolar dilation after acute hypertension. / Wei, E. P.; Kontos, H. A.; Christman, C. W.; Dewitt, Douglas; Povlishock, J. T.

In: Circulation Research, Vol. 57, No. 5, 1985, p. 781-787.

Research output: Contribution to journalArticle

Wei, E. P. ; Kontos, H. A. ; Christman, C. W. ; Dewitt, Douglas ; Povlishock, J. T. / Superoxide generation and reversal of acetylcholine-induced cerebral arteriolar dilation after acute hypertension. In: Circulation Research. 1985 ; Vol. 57, No. 5. pp. 781-787.
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