Suppression of angiotensin II stimulated responses in aortic vascular smooth muscle cells of experimental cirrhotic rats

Ru Zhang, Guo Liang Wang, Pi Li Zhang, Ying Xiong, Wen Bo Zhang, Xing Peng Wang, De Ling Yin, Qing Jing

Research output: Contribution to journalArticle

Abstract

Functional responses to angiotensin II (AT-II) were determined in aortic vascular smooth muscle cells (VSMCs) from experimental cirrhotic rats. Our data showed that AT-II-stimulated extracellular acidification rate (ECAR), which was measured by Cytosensor microphysiometry, was significantly reduced in the aortic VSMCs from the cirrhotic rats as compared to those from the control animals. The ability of AT-II to promote formation of inositol phosphates, the second messenger produced by the activation of Gq-coupled receptors, was also considerably suppressed in the cirrhotic VSMCs. Furthermore, the maximal p42/44 MAPK phosphorylation stimulated by AT-II was significantly reduced in the cirrhotic VSMCs in contrast to that in the normal VSMCs. Taken together, our data clearly demonstrated that the functional responses to AT-II was severely suppressed in aortic VSMCs in cirrhosis, indicating the impairment of general Gq-coupled receptor signaling and subsequent biological function in the cirrhotic VSMCs.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalCell Research
Volume9
Issue number2
DOIs
StatePublished - Jun 1999
Externally publishedYes

Keywords

  • Angiotensin II
  • Aortic vascular smooth muscle cells
  • Cirrhosis
  • Receptor responses

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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