Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses

Yukinobu Tohya, Krishna Narayanan, Wataru Kamitani, Cheng Huang, Kumari Lokugamage, Shinji Makino

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

nsp1 protein of severe acute respiratory syndrome coronavirus (SARS-CoV), a group 2b CoV, suppresses host gene expression by promoting host mRNA degradation and translation inhibition. The present study analyzed the activities of nsp1 proteins from the group 2 bat CoV strains Rm1, 133, and HKU9-1, belonging to groups 2b, 2c, and 2d, respectively. The host mRNA degradation and translational suppression activities of nsp1 of SARS-CoV and Rm1 nsp1 were similar and stronger than the activities of the nsp1 proteins of 133 and HKU9-1. Rm1 nsp1 expression in trans strongly inhibited the induction of type I interferon (IFN-I) and IFN-stimulated genes in cells infected with an IFN-inducing SARS-CoV mutant, while 133 and HKU9-1 nsp1 proteins had relatively moderate IFN-inhibitory activities. The results of our studies suggested a conserved function among nsp1 proteins of SARS-CoV and group 2 bat CoVs.

Original languageEnglish (US)
Pages (from-to)5282-5288
Number of pages7
JournalJournal of Virology
Volume83
Issue number10
DOIs
StatePublished - May 2009

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Coronavirus
Coronavirinae
Severe Acute Respiratory Syndrome
Chiroptera
Gene Expression
gene expression
RNA Stability
Proteins
proteins
Interferon Type I
degradation
Protein Biosynthesis
interferons
translation (genetics)
mutants
Severe acute respiratory syndrome coronavirus
Genes
genes
cells

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses. / Tohya, Yukinobu; Narayanan, Krishna; Kamitani, Wataru; Huang, Cheng; Lokugamage, Kumari; Makino, Shinji.

In: Journal of Virology, Vol. 83, No. 10, 05.2009, p. 5282-5288.

Research output: Contribution to journalArticle

Tohya, Yukinobu ; Narayanan, Krishna ; Kamitani, Wataru ; Huang, Cheng ; Lokugamage, Kumari ; Makino, Shinji. / Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses. In: Journal of Virology. 2009 ; Vol. 83, No. 10. pp. 5282-5288.
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