Suppression of human synovial cell proliferation by dihomo-γ-linolenic acid

D. G. Baker, K. A. Krakauer, G. Tate, Michael Laposata, R. B. Zurier

Research output: Contribution to journalArticle

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Abstract

Prostaglandin E1 (PGE1) and oils enriched in its precursor fatty acids suppress inflammation and joint tissue injury in several animal models. Since synovial cell proliferation is a hallmark of rheumatoid arthritis, we studied the effect of dihomo-γ-linolenic acid (DGLA), an immediate precursor of PGE1, on the growth of human adherent synovial cells (ASC) in tissue culture. When stimulated by appropriate concentrations of recombinant interleukin-1β (rIL-1β), ASC proliferate and produce PGE. DGLA-enriched medium suppressed both baseline and rIL-1β-stimulated ASC growth fivefold, compared with medium supplemented with arachidonic acid. Indomethacin reduced the effect of the DGLA. Synovial cells incorporated the DGLA, and rIL-1β-stimulated cells that were incubated with DGLA exhibited a 14-fold increase in PGE1 (to 25.2 ± 6.0 ng/ml, mean ± SD) and a 70% decrease in PGE2 (to 25.2 ± 4.2 ng/ml) compared with cells in control medium. At equivalent concentrations (5 x 10-7 M), PGE1 increased the level of cellular cAMP to a greater extent than did PGE2 (16.8 ± 2.0 pmoles versus 4.3 ± 1.9 pmoles, mean ± SEM). Exogenous PGE1 was also a more effective inhibitor of cell growth. Similarly, cAMP concentrations in cells exposed to DGLA for 6 hours were greater than concentrations in arachidonic acid-enriched cultures (17.8 ± 3.3 pmoles versus 2.1 ± 2.0 pmoles). These observations suggest that DGLA can restrain ASC growth, an effect which may be due to its capacity to increase PGE1 production and subsequent cellular cAMP concentration.

Original languageEnglish (US)
Pages (from-to)1273-1281
Number of pages9
JournalArthritis and Rheumatism
Volume32
Issue number10
StatePublished - 1989
Externally publishedYes

Fingerprint

alpha-Linolenic Acid
Alprostadil
Cell Proliferation
Interleukin-1
Dinoprostone
Arachidonic Acid
Growth
Growth Inhibitors
Prostaglandins E
Indomethacin
Rheumatoid Arthritis
Oils
Fatty Acids
Animal Models
Joints
Inflammation
Wounds and Injuries

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Baker, D. G., Krakauer, K. A., Tate, G., Laposata, M., & Zurier, R. B. (1989). Suppression of human synovial cell proliferation by dihomo-γ-linolenic acid. Arthritis and Rheumatism, 32(10), 1273-1281.

Suppression of human synovial cell proliferation by dihomo-γ-linolenic acid. / Baker, D. G.; Krakauer, K. A.; Tate, G.; Laposata, Michael; Zurier, R. B.

In: Arthritis and Rheumatism, Vol. 32, No. 10, 1989, p. 1273-1281.

Research output: Contribution to journalArticle

Baker, DG, Krakauer, KA, Tate, G, Laposata, M & Zurier, RB 1989, 'Suppression of human synovial cell proliferation by dihomo-γ-linolenic acid', Arthritis and Rheumatism, vol. 32, no. 10, pp. 1273-1281.
Baker, D. G. ; Krakauer, K. A. ; Tate, G. ; Laposata, Michael ; Zurier, R. B. / Suppression of human synovial cell proliferation by dihomo-γ-linolenic acid. In: Arthritis and Rheumatism. 1989 ; Vol. 32, No. 10. pp. 1273-1281.
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