We studied the effect of human tumor cells grown in short term cultures on the proliferative response of normal human peripheral blood mononuclear cells (PBMC) to mitogens and alloantigens. In 6 of 7 short term cancer cell lines studied, the addition of 103 cells to mitogen- or alloantigen-stimulated cultures of 105 PBMC caused from 20 to 60% suppression of 3H thymidine incorporation. Cells from short term cultures established from biopsies of normal skin did not suppress at these concentrations. The suppression was not due to a change in kinetics, or to a decrease in viability or recovery of either the tumor cells or PBMC in the cultures. Prior treatment of tumor cells with mitomycin C abrogated the suppression, while prior X-irradiation with 1,000, 2,000 or 3,000 rads had no effect. One of the tumor cells caused suppression when separated from the PBMC by a cell-impermeable membrane while another line required cell-to-cell contact for suppression.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Clinical and Laboratory Immunology|
|State||Published - 1981|
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