Suppression of the temperature-sensitive character of adenovirus 12 early mutants in monkey cells transformed by an adenovirus 7-simian virus 40 hybrid

Kazuko Shiroki, Hiroto Shimojo, Kenji Sekikawa, Kei Fujinaga, Jeffrey Rabek, Arnold J. Levine

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Temperature-sensitive mutants of adenovirus 12, defective in viral DNA replication, fall into three complementation groups; tsA, tsB and tsC. Each of these classes was temperature dependent for virus production, viral DNA synthesis and virion antigen in human embryonic kidney cells and in T22 cells, a monkey cell line transformed by defective particles of simian virus 40 (SV40). In H5 cells, a monkey cell line transformed by an adenovirus 7-SV40 hybrid, however, the tsB and tsC mutants were not temperature sensitive for viral replication while the tsA mutant retained its temperature-sensitive phenotype. Wild-type adenovirus 7, but not wild-type SV40, was able to complement tsA, tsB and tsC for either infectious virus production or DNA replication. These observations suggest that adenovirus 7-specific functions present in the H5 cells complement or suppress the tsB and tsC gene functions. DNA-DNA reassociation kinetics and DNA-RNA hybridization competition experiments have been performed and demonstrate the presence and the transcription of at least a portion of the adenovirus 7 genome in the H5 cells.

Original languageEnglish (US)
Pages (from-to)431-437
Number of pages7
JournalVirology
Volume69
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Simian virus 40
Adenoviridae
Haplorhini
Temperature
Transformed Cell Line
Viral DNA
DNA Replication
DNA
Virus Replication
Virion
Genome
RNA
Viruses
Phenotype
Kidney
Antigens
Genes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Suppression of the temperature-sensitive character of adenovirus 12 early mutants in monkey cells transformed by an adenovirus 7-simian virus 40 hybrid. / Shiroki, Kazuko; Shimojo, Hiroto; Sekikawa, Kenji; Fujinaga, Kei; Rabek, Jeffrey; Levine, Arnold J.

In: Virology, Vol. 69, No. 2, 1976, p. 431-437.

Research output: Contribution to journalArticle

Shiroki, Kazuko ; Shimojo, Hiroto ; Sekikawa, Kenji ; Fujinaga, Kei ; Rabek, Jeffrey ; Levine, Arnold J. / Suppression of the temperature-sensitive character of adenovirus 12 early mutants in monkey cells transformed by an adenovirus 7-simian virus 40 hybrid. In: Virology. 1976 ; Vol. 69, No. 2. pp. 431-437.
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AU - Levine, Arnold J.

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