Suppression of tumorigenesis in the Apcmin mouse: Down-regulation of β-catenin signaling by a combination of tea plus sulindac

Gayle A. Orner, W. Mohaiza Dashwood, Carmen A. Blum, G. Darío Díaz, Qingjie Li, Roderick H. Dashwood

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Epidemiological and animal studies suggest that tea may be protective towards cancers of the GI tract. White tea, the least processed form of tea, contains high levels of polyphenols and, like green tea, is chemopreventive towards heterocyclic amine-initiated colonic aberrant crypt formation in male F344 rats. We examined for the first time the relative effectiveness of white and green tea in suppressing intestinal tumorigenesis in C57BL/6J-ApcMin/+ (Apcmin) mice. Each tea was also compared with sulindac, a nonsteroidal anti-inflammatory drug known to be highly effective in Apcmin mice. Male C57BL/6J +/+ (wild-type) and Apcmin mice were treated in the drinking water with white tea or green tea (1.5% w/v, 2 min brew-time), 80 p.p.m. sulindac, a combination of 80 p.p.m. sulindac in 1.5% white tea, or pH buffered water. After 12 weeks of treatment, Apcmin mice given white tea, green tea, or sulindac had significantly fewer tumors than controls (P < 0.05). The protection provided by 1.5% green or white tea was comparable to that provided by 80 p.p.m. sulindac. Mice treated with a combination of white tea plus sulindac had significantly fewer tumors than either treatment alone (P < 0.05). β-catenin and β-catenin/Tcf-4 regulated proteins Cyclin D1 and c-Jun were readily detected in polyps, but markedly reduced in normal-looking intestines of mice treated with both tea and sulindac. This research provides evidence that teas, particularly when administered in combination with sulindac, are highly effective at inhibiting intestinal neoplasia in male Apcmin mice via direct or indirect effects on the β-catenin/APC pathway.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalCarcinogenesis
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Sulindac
Catenins
Tea
Carcinogenesis
Down-Regulation
Neoplasms
Cyclin D1
Inbred F344 Rats
Polyphenols

ASJC Scopus subject areas

  • Cancer Research

Cite this

Suppression of tumorigenesis in the Apcmin mouse : Down-regulation of β-catenin signaling by a combination of tea plus sulindac. / Orner, Gayle A.; Dashwood, W. Mohaiza; Blum, Carmen A.; Díaz, G. Darío; Li, Qingjie; Dashwood, Roderick H.

In: Carcinogenesis, Vol. 24, No. 2, 01.02.2003, p. 263-267.

Research output: Contribution to journalArticle

Orner, Gayle A. ; Dashwood, W. Mohaiza ; Blum, Carmen A. ; Díaz, G. Darío ; Li, Qingjie ; Dashwood, Roderick H. / Suppression of tumorigenesis in the Apcmin mouse : Down-regulation of β-catenin signaling by a combination of tea plus sulindac. In: Carcinogenesis. 2003 ; Vol. 24, No. 2. pp. 263-267.
@article{7822e99738d54ae8bab6fca0816b9c57,
title = "Suppression of tumorigenesis in the Apcmin mouse: Down-regulation of β-catenin signaling by a combination of tea plus sulindac",
abstract = "Epidemiological and animal studies suggest that tea may be protective towards cancers of the GI tract. White tea, the least processed form of tea, contains high levels of polyphenols and, like green tea, is chemopreventive towards heterocyclic amine-initiated colonic aberrant crypt formation in male F344 rats. We examined for the first time the relative effectiveness of white and green tea in suppressing intestinal tumorigenesis in C57BL/6J-ApcMin/+ (Apcmin) mice. Each tea was also compared with sulindac, a nonsteroidal anti-inflammatory drug known to be highly effective in Apcmin mice. Male C57BL/6J +/+ (wild-type) and Apcmin mice were treated in the drinking water with white tea or green tea (1.5{\%} w/v, 2 min brew-time), 80 p.p.m. sulindac, a combination of 80 p.p.m. sulindac in 1.5{\%} white tea, or pH buffered water. After 12 weeks of treatment, Apcmin mice given white tea, green tea, or sulindac had significantly fewer tumors than controls (P < 0.05). The protection provided by 1.5{\%} green or white tea was comparable to that provided by 80 p.p.m. sulindac. Mice treated with a combination of white tea plus sulindac had significantly fewer tumors than either treatment alone (P < 0.05). β-catenin and β-catenin/Tcf-4 regulated proteins Cyclin D1 and c-Jun were readily detected in polyps, but markedly reduced in normal-looking intestines of mice treated with both tea and sulindac. This research provides evidence that teas, particularly when administered in combination with sulindac, are highly effective at inhibiting intestinal neoplasia in male Apcmin mice via direct or indirect effects on the β-catenin/APC pathway.",
author = "Orner, {Gayle A.} and Dashwood, {W. Mohaiza} and Blum, {Carmen A.} and D{\'i}az, {G. Dar{\'i}o} and Qingjie Li and Dashwood, {Roderick H.}",
year = "2003",
month = "2",
day = "1",
doi = "10.1093/carcin/24.2.263",
language = "English (US)",
volume = "24",
pages = "263--267",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Suppression of tumorigenesis in the Apcmin mouse

T2 - Down-regulation of β-catenin signaling by a combination of tea plus sulindac

AU - Orner, Gayle A.

AU - Dashwood, W. Mohaiza

AU - Blum, Carmen A.

AU - Díaz, G. Darío

AU - Li, Qingjie

AU - Dashwood, Roderick H.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Epidemiological and animal studies suggest that tea may be protective towards cancers of the GI tract. White tea, the least processed form of tea, contains high levels of polyphenols and, like green tea, is chemopreventive towards heterocyclic amine-initiated colonic aberrant crypt formation in male F344 rats. We examined for the first time the relative effectiveness of white and green tea in suppressing intestinal tumorigenesis in C57BL/6J-ApcMin/+ (Apcmin) mice. Each tea was also compared with sulindac, a nonsteroidal anti-inflammatory drug known to be highly effective in Apcmin mice. Male C57BL/6J +/+ (wild-type) and Apcmin mice were treated in the drinking water with white tea or green tea (1.5% w/v, 2 min brew-time), 80 p.p.m. sulindac, a combination of 80 p.p.m. sulindac in 1.5% white tea, or pH buffered water. After 12 weeks of treatment, Apcmin mice given white tea, green tea, or sulindac had significantly fewer tumors than controls (P < 0.05). The protection provided by 1.5% green or white tea was comparable to that provided by 80 p.p.m. sulindac. Mice treated with a combination of white tea plus sulindac had significantly fewer tumors than either treatment alone (P < 0.05). β-catenin and β-catenin/Tcf-4 regulated proteins Cyclin D1 and c-Jun were readily detected in polyps, but markedly reduced in normal-looking intestines of mice treated with both tea and sulindac. This research provides evidence that teas, particularly when administered in combination with sulindac, are highly effective at inhibiting intestinal neoplasia in male Apcmin mice via direct or indirect effects on the β-catenin/APC pathway.

AB - Epidemiological and animal studies suggest that tea may be protective towards cancers of the GI tract. White tea, the least processed form of tea, contains high levels of polyphenols and, like green tea, is chemopreventive towards heterocyclic amine-initiated colonic aberrant crypt formation in male F344 rats. We examined for the first time the relative effectiveness of white and green tea in suppressing intestinal tumorigenesis in C57BL/6J-ApcMin/+ (Apcmin) mice. Each tea was also compared with sulindac, a nonsteroidal anti-inflammatory drug known to be highly effective in Apcmin mice. Male C57BL/6J +/+ (wild-type) and Apcmin mice were treated in the drinking water with white tea or green tea (1.5% w/v, 2 min brew-time), 80 p.p.m. sulindac, a combination of 80 p.p.m. sulindac in 1.5% white tea, or pH buffered water. After 12 weeks of treatment, Apcmin mice given white tea, green tea, or sulindac had significantly fewer tumors than controls (P < 0.05). The protection provided by 1.5% green or white tea was comparable to that provided by 80 p.p.m. sulindac. Mice treated with a combination of white tea plus sulindac had significantly fewer tumors than either treatment alone (P < 0.05). β-catenin and β-catenin/Tcf-4 regulated proteins Cyclin D1 and c-Jun were readily detected in polyps, but markedly reduced in normal-looking intestines of mice treated with both tea and sulindac. This research provides evidence that teas, particularly when administered in combination with sulindac, are highly effective at inhibiting intestinal neoplasia in male Apcmin mice via direct or indirect effects on the β-catenin/APC pathway.

UR - http://www.scopus.com/inward/record.url?scp=0037330524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037330524&partnerID=8YFLogxK

U2 - 10.1093/carcin/24.2.263

DO - 10.1093/carcin/24.2.263

M3 - Article

C2 - 12584176

AN - SCOPUS:0037330524

VL - 24

SP - 263

EP - 267

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 2

ER -