Supramedullary afferents of the nucleus raphe magnus in the rat: A study using the transcannula HRP gel and autoradiographic techniques

S. M. Carlton, G. R. Leichnetz, E. G. Young, D. J. Mayer

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    88 Citations (Scopus)

    Abstract

    Afferents of the nucleus raphe magnus (NRM) were retrogradely labelled by using a transcannula HRP gel technique in conjunction with tetramethylbenzidine neuerohistochemistry to determine the sources of inputs to the nucleus which could potentially influence the descending antinociceptive raphe-spinal system. Large numbers of HRP-labelled neurons were seen in the frontal cortex, dorsomedial nucleus of the hypothalamus, zona incerta, nucleus parafascicularis prerubralis (NPfPr), pretectum, dorsal and lateral periaqueductal gray, nucleus cuneiformis (NC), deep superior colliculus (dSC), a paraoculomotor cell group which may be the medial accessory nucleus of Bechterew, dorsal column nuclei, and spinal trigeminal nucleus. Smaller numbers of labelled cells were also observed in the preoptic area, nucleus of Darkschewitsch, ventral peri(third)ventricular gray, nucleus reticularis pontis oralis and caudalis, medial and lateral vestibular nuclei, and a subdivision of the hypoglossal nucleus. Confirmational anterograde autoradiographic studies were performed by injecting tritiated leucine into two of the principal sources of afferents to NRM: NPfPr, and dSC/NC. The results are compared with control HRP gel implants in the inferior olive, spinal cord, nucleus reticularis paragigantocellularis, and medial facial nucleus. Comments are also made concerning the parcellation of the ventromedial medulla and the possible role of both NRM and its afferents in central analgesic mechanisms.

    Original languageEnglish (US)
    Pages (from-to)43-58
    Number of pages16
    JournalJournal of Comparative Neurology
    Volume214
    Issue number1
    StatePublished - 1983

    Fingerprint

    Intralaminar Thalamic Nuclei
    Preoptic Area
    Superior Colliculi
    Gels
    Lateral Vestibular Nucleus
    Tegmentum Mesencephali
    Spinal Trigeminal Nucleus
    Mediodorsal Thalamic Nucleus
    Vestibular Nuclei
    Periaqueductal Gray
    Raphe Nuclei
    Frontal Lobe
    Leucine
    Hypothalamus
    Analgesics
    Spinal Cord
    Cell Count
    Neurons
    Nucleus Raphe Magnus
    Zona Incerta

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Supramedullary afferents of the nucleus raphe magnus in the rat : A study using the transcannula HRP gel and autoradiographic techniques. / Carlton, S. M.; Leichnetz, G. R.; Young, E. G.; Mayer, D. J.

    In: Journal of Comparative Neurology, Vol. 214, No. 1, 1983, p. 43-58.

    Research output: Contribution to journalArticle

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