Abstract
Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9136-9143 |
| Number of pages | 8 |
| Journal | ACS Omega |
| Volume | 2 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jan 1 2017 |
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ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells. / Rudra, Jai S.; Khan, Arshad; Clover, Tara M.; Endsley, Janice; Zloza, Andrew; Wang, Jin; Jagannath, Chinnaswamy.
In: ACS Omega, Vol. 2, No. 12, 01.01.2017, p. 9136-9143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells
AU - Rudra, Jai S.
AU - Khan, Arshad
AU - Clover, Tara M.
AU - Endsley, Janice
AU - Zloza, Andrew
AU - Wang, Jin
AU - Jagannath, Chinnaswamy
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.
AB - Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.
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U2 - 10.1021/acsomega.7b00525
DO - 10.1021/acsomega.7b00525
M3 - Article
VL - 2
SP - 9136
EP - 9143
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
IS - 12
ER -