Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells

Jai S. Rudra, Arshad Khan, Tara M. Clover, Janice Endsley, Andrew Zloza, Jin Wang, Chinnaswamy Jagannath

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.

Original languageEnglish (US)
Pages (from-to)9136-9143
Number of pages8
JournalACS Omega
Volume2
Issue number12
DOIs
StatePublished - Jan 1 2017

Fingerprint

Nanofibers
Peptides
Bearings (structural)
Proteins
Epitopes
T-Lymphocyte Epitopes
Microtubule-Associated Proteins
Vaccines
T-cells
Antigens
Modulators
Assays
Microscopic examination
Membranes
Antigen-Presenting Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Rudra, J. S., Khan, A., Clover, T. M., Endsley, J., Zloza, A., Wang, J., & Jagannath, C. (2017). Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells. ACS Omega, 2(12), 9136-9143. https://doi.org/10.1021/acsomega.7b00525

Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells. / Rudra, Jai S.; Khan, Arshad; Clover, Tara M.; Endsley, Janice; Zloza, Andrew; Wang, Jin; Jagannath, Chinnaswamy.

In: ACS Omega, Vol. 2, No. 12, 01.01.2017, p. 9136-9143.

Research output: Contribution to journalArticle

Rudra, JS, Khan, A, Clover, TM, Endsley, J, Zloza, A, Wang, J & Jagannath, C 2017, 'Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells', ACS Omega, vol. 2, no. 12, pp. 9136-9143. https://doi.org/10.1021/acsomega.7b00525
Rudra, Jai S. ; Khan, Arshad ; Clover, Tara M. ; Endsley, Janice ; Zloza, Andrew ; Wang, Jin ; Jagannath, Chinnaswamy. / Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells. In: ACS Omega. 2017 ; Vol. 2, No. 12. pp. 9136-9143.
@article{5060fc8989cd4b3281e3311bc193cb4a,
title = "Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells",
abstract = "Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.",
author = "Rudra, {Jai S.} and Arshad Khan and Clover, {Tara M.} and Janice Endsley and Andrew Zloza and Jin Wang and Chinnaswamy Jagannath",
year = "2017",
month = "1",
day = "1",
doi = "10.1021/acsomega.7b00525",
language = "English (US)",
volume = "2",
pages = "9136--9143",
journal = "ACS Omega",
issn = "2470-1343",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells

AU - Rudra, Jai S.

AU - Khan, Arshad

AU - Clover, Tara M.

AU - Endsley, Janice

AU - Zloza, Andrew

AU - Wang, Jin

AU - Jagannath, Chinnaswamy

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.

AB - Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins. The incorporation of microtubule-associated protein 1A/1B-light chain-3 (LC3-II) into the autophagosomal membrane, a key biological marker for autophagy, was confirmed using microscopy. Our findings indicate that autophagy in APCs plays an essential role in the mechanism of adjuvant action of supramolecular peptide nanofibers.

UR - http://www.scopus.com/inward/record.url?scp=85054546385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054546385&partnerID=8YFLogxK

U2 - 10.1021/acsomega.7b00525

DO - 10.1021/acsomega.7b00525

M3 - Article

AN - SCOPUS:85054546385

VL - 2

SP - 9136

EP - 9143

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 12

ER -