Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids

D. Patrick O'Neal, Massoud Motamedi, Jefferson Chen, Gerard L. Cote

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Traditional methods for the detection of excitatory amino acids, which have been linked to secondary injury following head trauma, can be excessively time consuming clinically. A near real-time measurement system could provide clinical information in anticipation of pharmaceutical intervention for head injured patients. Our studies have shown that Surface-Enhanced Raman Spectroscopy (SERS) with silver colloids has the ability to measure physiological concentrations of in vitro excitatory amino acids using short scan times. Employing a damage model for ischemia, preliminary ex vivo rat extracellular brain fluid analysis shows an intriguing correlation between SERS spectral features and expected Glutamate concentration fluctuations following head injuries.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages211-216
Number of pages6
Volume3608
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 Biomedical Applications of Raman Spectroscopy - San Jose, Ca, USA
Duration: Jan 25 1999Jan 26 1999

Other

OtherProceedings of the 1999 Biomedical Applications of Raman Spectroscopy
CitySan Jose, Ca, USA
Period1/25/991/26/99

Fingerprint

amino acids
Raman spectroscopy
Amino acids
ischemia
glutamates
Time measurement
Colloids
Drug products
rats
brain
colloids
Rats
Brain
Silver
silver
time measurement
damage
Fluids
fluids

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

O'Neal, D. P., Motamedi, M., Chen, J., & Cote, G. L. (1999). Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3608, pp. 211-216). Society of Photo-Optical Instrumentation Engineers.

Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids. / O'Neal, D. Patrick; Motamedi, Massoud; Chen, Jefferson; Cote, Gerard L.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608 Society of Photo-Optical Instrumentation Engineers, 1999. p. 211-216.

Research output: Chapter in Book/Report/Conference proceedingChapter

O'Neal, DP, Motamedi, M, Chen, J & Cote, GL 1999, Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3608, Society of Photo-Optical Instrumentation Engineers, pp. 211-216, Proceedings of the 1999 Biomedical Applications of Raman Spectroscopy, San Jose, Ca, USA, 1/25/99.
O'Neal DP, Motamedi M, Chen J, Cote GL. Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608. Society of Photo-Optical Instrumentation Engineers. 1999. p. 211-216
O'Neal, D. Patrick ; Motamedi, Massoud ; Chen, Jefferson ; Cote, Gerard L. / Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608 Society of Photo-Optical Instrumentation Engineers, 1999. pp. 211-216
@inbook{fcc9dec6bccd49f6aac1689fa343ea06,
title = "Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids",
abstract = "Traditional methods for the detection of excitatory amino acids, which have been linked to secondary injury following head trauma, can be excessively time consuming clinically. A near real-time measurement system could provide clinical information in anticipation of pharmaceutical intervention for head injured patients. Our studies have shown that Surface-Enhanced Raman Spectroscopy (SERS) with silver colloids has the ability to measure physiological concentrations of in vitro excitatory amino acids using short scan times. Employing a damage model for ischemia, preliminary ex vivo rat extracellular brain fluid analysis shows an intriguing correlation between SERS spectral features and expected Glutamate concentration fluctuations following head injuries.",
author = "O'Neal, {D. Patrick} and Massoud Motamedi and Jefferson Chen and Cote, {Gerard L.}",
year = "1999",
language = "English (US)",
volume = "3608",
pages = "211--216",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Society of Photo-Optical Instrumentation Engineers",

}

TY - CHAP

T1 - Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids

AU - O'Neal, D. Patrick

AU - Motamedi, Massoud

AU - Chen, Jefferson

AU - Cote, Gerard L.

PY - 1999

Y1 - 1999

N2 - Traditional methods for the detection of excitatory amino acids, which have been linked to secondary injury following head trauma, can be excessively time consuming clinically. A near real-time measurement system could provide clinical information in anticipation of pharmaceutical intervention for head injured patients. Our studies have shown that Surface-Enhanced Raman Spectroscopy (SERS) with silver colloids has the ability to measure physiological concentrations of in vitro excitatory amino acids using short scan times. Employing a damage model for ischemia, preliminary ex vivo rat extracellular brain fluid analysis shows an intriguing correlation between SERS spectral features and expected Glutamate concentration fluctuations following head injuries.

AB - Traditional methods for the detection of excitatory amino acids, which have been linked to secondary injury following head trauma, can be excessively time consuming clinically. A near real-time measurement system could provide clinical information in anticipation of pharmaceutical intervention for head injured patients. Our studies have shown that Surface-Enhanced Raman Spectroscopy (SERS) with silver colloids has the ability to measure physiological concentrations of in vitro excitatory amino acids using short scan times. Employing a damage model for ischemia, preliminary ex vivo rat extracellular brain fluid analysis shows an intriguing correlation between SERS spectral features and expected Glutamate concentration fluctuations following head injuries.

UR - http://www.scopus.com/inward/record.url?scp=0032681674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032681674&partnerID=8YFLogxK

M3 - Chapter

VL - 3608

SP - 211

EP - 216

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Society of Photo-Optical Instrumentation Engineers

ER -