Surface morphology of normal and neoplastic rat cells

M. W. Cloyd, D. D. Bigner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nontumorigenic rat cells and their tumorigenic counterparts were studied with scanning electron microscopy under controlled conditions in vitro and with transmission electron microscopy after replantation in vivo to discern if external morphology reflected the cell's neoplastic state or the etiology of transformation. Interphase cells in 6 of 7 nontumorigenic lines were flat and monolayered under confluent conditions and exhibited smooth, nonactive cell surfaces. A nontumorigenic cell line morphologically transformed with human adenovirus 2 consisted of spherical cells with blebbed surfaces. Cells from 6 tumorigenic lines transformed with avian sarcoma virus had highly active surfaces with many surface projections. Cells from 2 chemical carcinogen transformed rat embryo lines were flat with no surface projections in subconfluent culture and rounded with only a few microvilli at high densities, but cells from a sarcoma chemically induced in an adult rat were villous. When villous cells were syngeneically replanted in vivo, they lost most microvilli. The external morphology of cells was influenced by a number of factors simultaneously, with no universal pattern associated with tumorigenic capacity or transforming agent.

Original languageEnglish (US)
Pages (from-to)29-46
Number of pages18
JournalAmerican Journal of Pathology
Volume88
Issue number1
StatePublished - 1977

Fingerprint

Microvilli
Avian Sarcoma Viruses
Human Adenoviruses
Transformed Cell Line
Replantation
Interphase
Transmission Electron Microscopy
Sarcoma
Carcinogens
Electron Scanning Microscopy
Embryonic Structures
Cell Count
In Vitro Techniques

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cloyd, M. W., & Bigner, D. D. (1977). Surface morphology of normal and neoplastic rat cells. American Journal of Pathology, 88(1), 29-46.

Surface morphology of normal and neoplastic rat cells. / Cloyd, M. W.; Bigner, D. D.

In: American Journal of Pathology, Vol. 88, No. 1, 1977, p. 29-46.

Research output: Contribution to journalArticle

Cloyd, MW & Bigner, DD 1977, 'Surface morphology of normal and neoplastic rat cells', American Journal of Pathology, vol. 88, no. 1, pp. 29-46.
Cloyd, M. W. ; Bigner, D. D. / Surface morphology of normal and neoplastic rat cells. In: American Journal of Pathology. 1977 ; Vol. 88, No. 1. pp. 29-46.
@article{b72abc421c724b929dddec953082e0d0,
title = "Surface morphology of normal and neoplastic rat cells",
abstract = "Nontumorigenic rat cells and their tumorigenic counterparts were studied with scanning electron microscopy under controlled conditions in vitro and with transmission electron microscopy after replantation in vivo to discern if external morphology reflected the cell's neoplastic state or the etiology of transformation. Interphase cells in 6 of 7 nontumorigenic lines were flat and monolayered under confluent conditions and exhibited smooth, nonactive cell surfaces. A nontumorigenic cell line morphologically transformed with human adenovirus 2 consisted of spherical cells with blebbed surfaces. Cells from 6 tumorigenic lines transformed with avian sarcoma virus had highly active surfaces with many surface projections. Cells from 2 chemical carcinogen transformed rat embryo lines were flat with no surface projections in subconfluent culture and rounded with only a few microvilli at high densities, but cells from a sarcoma chemically induced in an adult rat were villous. When villous cells were syngeneically replanted in vivo, they lost most microvilli. The external morphology of cells was influenced by a number of factors simultaneously, with no universal pattern associated with tumorigenic capacity or transforming agent.",
author = "Cloyd, {M. W.} and Bigner, {D. D.}",
year = "1977",
language = "English (US)",
volume = "88",
pages = "29--46",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Surface morphology of normal and neoplastic rat cells

AU - Cloyd, M. W.

AU - Bigner, D. D.

PY - 1977

Y1 - 1977

N2 - Nontumorigenic rat cells and their tumorigenic counterparts were studied with scanning electron microscopy under controlled conditions in vitro and with transmission electron microscopy after replantation in vivo to discern if external morphology reflected the cell's neoplastic state or the etiology of transformation. Interphase cells in 6 of 7 nontumorigenic lines were flat and monolayered under confluent conditions and exhibited smooth, nonactive cell surfaces. A nontumorigenic cell line morphologically transformed with human adenovirus 2 consisted of spherical cells with blebbed surfaces. Cells from 6 tumorigenic lines transformed with avian sarcoma virus had highly active surfaces with many surface projections. Cells from 2 chemical carcinogen transformed rat embryo lines were flat with no surface projections in subconfluent culture and rounded with only a few microvilli at high densities, but cells from a sarcoma chemically induced in an adult rat were villous. When villous cells were syngeneically replanted in vivo, they lost most microvilli. The external morphology of cells was influenced by a number of factors simultaneously, with no universal pattern associated with tumorigenic capacity or transforming agent.

AB - Nontumorigenic rat cells and their tumorigenic counterparts were studied with scanning electron microscopy under controlled conditions in vitro and with transmission electron microscopy after replantation in vivo to discern if external morphology reflected the cell's neoplastic state or the etiology of transformation. Interphase cells in 6 of 7 nontumorigenic lines were flat and monolayered under confluent conditions and exhibited smooth, nonactive cell surfaces. A nontumorigenic cell line morphologically transformed with human adenovirus 2 consisted of spherical cells with blebbed surfaces. Cells from 6 tumorigenic lines transformed with avian sarcoma virus had highly active surfaces with many surface projections. Cells from 2 chemical carcinogen transformed rat embryo lines were flat with no surface projections in subconfluent culture and rounded with only a few microvilli at high densities, but cells from a sarcoma chemically induced in an adult rat were villous. When villous cells were syngeneically replanted in vivo, they lost most microvilli. The external morphology of cells was influenced by a number of factors simultaneously, with no universal pattern associated with tumorigenic capacity or transforming agent.

UR - http://www.scopus.com/inward/record.url?scp=0017639789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017639789&partnerID=8YFLogxK

M3 - Article

VL - 88

SP - 29

EP - 46

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 1

ER -