Surfactosomes: A novel approach to the reconstitution and 2-D crystallisation of membrane proteins

Ilya Reviakine, Svetla Stoylova, Andreas Holzenburg

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution, and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H+-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a = b = 13.0 ± 0.2 nm with γ = 90° and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.

Original languageEnglish (US)
Pages (from-to)296-300
Number of pages5
JournalFEBS Letters
Volume380
Issue number3
DOIs
StatePublished - Feb 19 1996

Keywords

  • 2-D crystallisation
  • Electron microscopy
  • Membrane protein
  • Reconstitution
  • Structure
  • Vacuolar H-ATPase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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