Surfactosomes: A novel approach to the reconstitution and 2-D crystallisation of membrane proteins

Ilya Reviakine; Svetla Stoylova; Andreas Holzenburg

doi:10.1016/0014-5793(96)00063-4

Surfactosomes: A novel approach to the reconstitution and 2-D crystallisation of membrane proteins

Ilya Reviakine, Svetla Stoylova, Andreas Holzenburg

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution, and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H⁺-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a = b = 13.0 ± 0.2 nm with γ = 90° and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.

Original language	English (US)
Pages (from-to)	296-300
Number of pages	5
Journal	FEBS Letters
Volume	380
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(96)00063-4
State	Published - Feb 19 1996
Externally published	Yes

Keywords

2-D crystallisation
Electron microscopy
Membrane protein
Reconstitution
Structure
Vacuolar H-ATPase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(96)00063-4

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abstract = "The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution, and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H+-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a = b = 13.0 ± 0.2 nm with γ = 90° and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.",

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author = "Ilya Reviakine and Svetla Stoylova and Andreas Holzenburg",

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T2 - A novel approach to the reconstitution and 2-D crystallisation of membrane proteins

AU - Reviakine, Ilya

AU - Stoylova, Svetla

AU - Holzenburg, Andreas

PY - 1996/2/19

Y1 - 1996/2/19

N2 - The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution, and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H+-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a = b = 13.0 ± 0.2 nm with γ = 90° and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.

AB - The formation of vesicle-like structures (termed surfactosomes) and lamellar sheets from solutions containing ammonium perfluoroocanoate (APFO) is illustrated using conventional and cryo-transmission electron microscopy. It is shown how this detergent can be used for the solubilisation, reconstitution, and 2-D crystallisation of membrane proteins as demonstrated for the major protein of the membrane sector of the V-type H+-ATPase (16-kDa protein). Electron microscopical analysis of 2-D crystals of the 16-kDa protein (a = b = 13.0 ± 0.2 nm with γ = 90° and p4 projection symmetry) revealed a unit cell comprising four dimeric complexes of the 16-kDa protein the significance of which is discussed.

KW - 2-D crystallisation

KW - Electron microscopy

KW - Membrane protein

KW - Reconstitution

KW - Structure

KW - Vacuolar H-ATPase

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JO - FEBS Letters

JF - FEBS Letters

IS - 3

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Surfactosomes: A novel approach to the reconstitution and 2-D crystallisation of membrane proteins

Abstract

Keywords

ASJC Scopus subject areas

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