Survival of mammary epithelial cells from virgin female BALB/c mice following in vivo gamma irradiation

L. M. Adams, S. P. Ethier, R. L. Ullrich

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To examine the cytotoxic effects of ionizing radiation on mouse mammary epithelial cells, a transplantation assay was used to generate a single-dose survival curve. In these experiments, virgin female BALB/c mice were irradiated with 0, 100, 300, 600, 900, 1200, or 1500 rad whole-body 137Cs radiation. Mammary glands of control and irradiated mice were removed and the mammary epithelial cells were enzymatically dissociated, either immediately or 24 hr following irradiation, serially diluted, and injected into gland-free mammary fat pads of 3 1/2 -week-old virgin female BALB/c host mice. The number of cells required to give ductal growth at 10 weeks in half the fat pads (the mammary cell dose 50%, MCD50) was determined by a maximum likelihood procedure and the ratio of MCD50 for nonirradiated control cells to MCD50 for irradiated cells provided an estimate of cell survival. Fitting the data using a single-hit multitarget model resulted in an estimate for D0 of 250 rad with an n of 2.4. No significant difference in the results was obtained whether the cells were dissociated and transplanted immediately after irradiation or the cells were allowed to remain in situ for 24 hr following irradiation. Thus no 'in situ repair' was detected for mouse mammary epithelial cells.

Original languageEnglish (US)
Pages (from-to)264-272
Number of pages9
JournalRadiation Research
Volume100
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

virgin females
breasts
gamma radiation
mice
Breast
epithelial cells
Epithelial Cells
irradiation
cells
dosage
fats
Human Mammary Glands
Adipose Tissue
mammary glands
transplantation
glands
Whole-Body Irradiation
Cell Transplantation
cell transplantation
estimates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Survival of mammary epithelial cells from virgin female BALB/c mice following in vivo gamma irradiation. / Adams, L. M.; Ethier, S. P.; Ullrich, R. L.

In: Radiation Research, Vol. 100, No. 2, 1984, p. 264-272.

Research output: Contribution to journalArticle

Adams, L. M. ; Ethier, S. P. ; Ullrich, R. L. / Survival of mammary epithelial cells from virgin female BALB/c mice following in vivo gamma irradiation. In: Radiation Research. 1984 ; Vol. 100, No. 2. pp. 264-272.
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