Susceptibility of thermally injured mice to cytomegalovirus infection

Hiroyuki Kobayashi, Makiko Kobayashi, David Herndon, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Thermally injured patients are very susceptible to infection with cytomegaloviruses. In this study a role of burn-associated type 2 T cell responses on the cytomegalovirus infection was examined in a mouse model of thermal injury. A predominance of type 2 T cell responses in splenic lymphocytes of thermally injured mice has been previously demonstrated. SCID mice inoculated with splenic T cells from thermally injured mice were susceptible to infection with a small amount (5 PFU/mouse) of murine cytomegalovirus (MCMV). Conversely, SCID mice inoculated with splenic T cells from normal mice were resistant to the same infection. High levels of IL-4 and IL-10, but not IFN-γ and IL-2, were detected in sera of thermally injured mice (TI-mice) infected with MCMV when those were compared with sera of normal mice infected with MCMV. IL-4 and IL-10 (type 2 cytokines) were produced by splenic T cells from MCMV-infected TI-mice, when they were stimulated in vitro with anti-CD3 mAb. Type 1 cytokines (IFN-γ and IL-2), however, were not produced by these T cells after the same stimulation. In contrast, splenic T cells from MCMV-infected normal mice produced type 1 cytokines by the stimulation with anti-CD3 mAb. These results suggest that the susceptibility of mice to MCMV infection is markedly influenced by burn-associated type 2 T cell responses.

Original languageEnglish (US)
Pages (from-to)675-680
Number of pages6
JournalBurns
Volume27
Issue number7
DOIs
StatePublished - 2001

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Muromegalovirus
Cytomegalovirus Infections
T-Lymphocytes
Interleukin-2
SCID Mice
Cytokines
Burns
Interleukin-4
Interleukin-10
Infection
Serum
Hot Temperature
Lymphocytes

Keywords

  • Burns
  • Cytomegalovirus
  • Type 2 T cells

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Susceptibility of thermally injured mice to cytomegalovirus infection. / Kobayashi, Hiroyuki; Kobayashi, Makiko; Herndon, David; Pollard, Richard B.; Suzuki, Fujio.

In: Burns, Vol. 27, No. 7, 2001, p. 675-680.

Research output: Contribution to journalArticle

Kobayashi, H, Kobayashi, M, Herndon, D, Pollard, RB & Suzuki, F 2001, 'Susceptibility of thermally injured mice to cytomegalovirus infection', Burns, vol. 27, no. 7, pp. 675-680. https://doi.org/10.1016/S0305-4179(01)00028-6
Kobayashi, Hiroyuki ; Kobayashi, Makiko ; Herndon, David ; Pollard, Richard B. ; Suzuki, Fujio. / Susceptibility of thermally injured mice to cytomegalovirus infection. In: Burns. 2001 ; Vol. 27, No. 7. pp. 675-680.
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