TY - JOUR
T1 - Susceptibility to SARS-CoV-2 of cell lines and substrates commonly used to diagnose and isolate influenza and other viruses
AU - Wang, Li
AU - Fan, Xiaoyu
AU - Bonenfant, Gaston
AU - Cui, Dan
AU - Hossain, Jaber
AU - Jiang, Nannan
AU - Larson, Gloria
AU - Currier, Michael
AU - Liddell, Jimma
AU - Wilson, Malania
AU - Tamin, Azaibi
AU - Harcourt, Jennifer
AU - Ciomperlik-Patton, Jessica
AU - Pang, Hong
AU - Dybdahl-Sissoko, Naomi
AU - Campagnoli, Ray
AU - Shi, Pei Yong
AU - Barnes, John
AU - Thornburg, Natalie J.
AU - Wentworth, David E.
AU - Zhou, Bin
N1 - Publisher Copyright:
© 2021 Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2021/5
Y1 - 2021/5
N2 - Co-infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viruses has been reported. We evaluated cell lines commonly used to isolate viruses and diagnose related diseases for their susceptibility to SARS-CoV-2. Although multiple kidney cell lines from monkeys were susceptible to SARS-CoV-2, we found many cell types derived from humans, dogs, minks, cats, mice, and chicken were not. We analyzed MDCK cells, which are most commonly used for surveillance and study of influenza viruses, and found that they were not susceptible to SARS-CoV-2. The low expression level of the angiotensin converting enzyme 2 receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by overexpression of canine angiotensin converting enzyme 2 in trans, strengthened the cellular barrier to productive infection. Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. Our findings should help avert inadvertent propagation of SARS-CoV-2 from diagnostic cell lines.
AB - Co-infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viruses has been reported. We evaluated cell lines commonly used to isolate viruses and diagnose related diseases for their susceptibility to SARS-CoV-2. Although multiple kidney cell lines from monkeys were susceptible to SARS-CoV-2, we found many cell types derived from humans, dogs, minks, cats, mice, and chicken were not. We analyzed MDCK cells, which are most commonly used for surveillance and study of influenza viruses, and found that they were not susceptible to SARS-CoV-2. The low expression level of the angiotensin converting enzyme 2 receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by overexpression of canine angiotensin converting enzyme 2 in trans, strengthened the cellular barrier to productive infection. Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. Our findings should help avert inadvertent propagation of SARS-CoV-2 from diagnostic cell lines.
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U2 - 10.3201/eid2705.210023
DO - 10.3201/eid2705.210023
M3 - Article
C2 - 33900165
AN - SCOPUS:85105480770
SN - 1080-6040
VL - 27
SP - 1380
EP - 1392
JO - Emerging infectious diseases
JF - Emerging infectious diseases
IS - 5
ER -