TY - JOUR
T1 - Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels
AU - Sherlock, Tim
AU - Nasrullah, Azeem
AU - Litvinov, Julia
AU - Cacao, Eliedonna
AU - Knoop, Jennifer
AU - Kemper, Steven
AU - Kourentzi, Katerina
AU - Kar, Archana
AU - Ruchhoeft, Paul
AU - Willson, Richard
N1 - Funding Information:
The project described was supported by Grant No. U54 AI057156 from NIAID/NIH. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the RCE Programs Office, NIAID, or NIH. Support was also provided by the National Science Foundation, Grant No. CMMI-0900743, the Alliance for Nanohealth Competitive Research Program (Grant No. W81XWH-09-2-0139), and the Welch Foundation, Grant No. E-1264. We also thank the students who participated in developing this project through the NSF Research Experience for Undergraduates program and the University of Houston (Project No. EEC-0647775), especially Ward Jablonski, Jon Elizalde, and David Shakarisaz. The authors thank the Center for Integrated Bio and Nanosystems and the University of Houston Nanofabrication Facility for providing excellent facilities and support.
PY - 2011/11
Y1 - 2011/11
N2 - Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.
AB - Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.
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U2 - 10.1116/1.3656801
DO - 10.1116/1.3656801
M3 - Article
C2 - 25530695
AN - SCOPUS:84255172520
SN - 2166-2746
VL - 29
JO - Journal of Vacuum Science and Technology B
JF - Journal of Vacuum Science and Technology B
IS - 6
M1 - 06FA01
ER -