Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels

Tim Sherlock, Azeem Nasrullah, Julia Litvinov, Eliedonna Cacao, Jennifer Knoop, Steven Kemper, Katerina Kourentzi, Archana Kar, Paul Ruchhoeft, Richard Willson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.

Original languageEnglish (US)
Article number06FA01
JournalJournal of Vacuum Science and Technology B:Nanotechnology and Microelectronics
Volume29
Issue number6
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

retroreflectors
Labels
Gold
Bearings (structural)
gold
proteins
Proteins
labor
numerical aperture
antibodies
Antibodies
Suspensions
platforms
Personnel
Magnetic fields
preparation
magnetic fields
Costs

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels. / Sherlock, Tim; Nasrullah, Azeem; Litvinov, Julia; Cacao, Eliedonna; Knoop, Jennifer; Kemper, Steven; Kourentzi, Katerina; Kar, Archana; Ruchhoeft, Paul; Willson, Richard.

In: Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics, Vol. 29, No. 6, 06FA01, 2011.

Research output: Contribution to journalArticle

Sherlock, T, Nasrullah, A, Litvinov, J, Cacao, E, Knoop, J, Kemper, S, Kourentzi, K, Kar, A, Ruchhoeft, P & Willson, R 2011, 'Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels', Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics, vol. 29, no. 6, 06FA01. https://doi.org/10.1116/1.3656801
Sherlock, Tim ; Nasrullah, Azeem ; Litvinov, Julia ; Cacao, Eliedonna ; Knoop, Jennifer ; Kemper, Steven ; Kourentzi, Katerina ; Kar, Archana ; Ruchhoeft, Paul ; Willson, Richard. / Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels. In: Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics. 2011 ; Vol. 29, No. 6.
@article{6c50512cc3084ee98f4b3bbc0f76ef1b,
title = "Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels",
abstract = "Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.",
author = "Tim Sherlock and Azeem Nasrullah and Julia Litvinov and Eliedonna Cacao and Jennifer Knoop and Steven Kemper and Katerina Kourentzi and Archana Kar and Paul Ruchhoeft and Richard Willson",
year = "2011",
doi = "10.1116/1.3656801",
language = "English (US)",
volume = "29",
journal = "Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics",
issn = "2166-2746",
publisher = "AVS Science and Technology Society",
number = "6",

}

TY - JOUR

T1 - Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels

AU - Sherlock, Tim

AU - Nasrullah, Azeem

AU - Litvinov, Julia

AU - Cacao, Eliedonna

AU - Knoop, Jennifer

AU - Kemper, Steven

AU - Kourentzi, Katerina

AU - Kar, Archana

AU - Ruchhoeft, Paul

AU - Willson, Richard

PY - 2011

Y1 - 2011

N2 - Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.

AB - Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.

UR - http://www.scopus.com/inward/record.url?scp=84255172520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84255172520&partnerID=8YFLogxK

U2 - 10.1116/1.3656801

DO - 10.1116/1.3656801

M3 - Article

AN - SCOPUS:84255172520

VL - 29

JO - Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics

JF - Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics

SN - 2166-2746

IS - 6

M1 - 06FA01

ER -