Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer

K. Yang; F. Faustinella; J. J. Xue; J. Whitson; A. Kampfl; X. S. Mu; X. Zhao; G. Taglialatela; J. R. Perez-Polo; G. Clifton; R. L. Hayes

doi:10.1016/0304-3940(94)90819-2

Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer

K. Yang, F. Faustinella, J. J. Xue, J. Whitson, A. Kampfl, X. S. Mu, X. Zhao, G. Taglialatela, J. R. Perez-Polo, G. Clifton, R. L. Hayes

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Abstract

We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.

Original language	English (US)
Pages (from-to)	291-294
Number of pages	4
Journal	Neuroscience Letters
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(94)90819-2
State	Published - Dec 5 1994

Keywords

Gene transfer
Liposome
NGF
Septo-hippocampal cell culture

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(94)90819-2

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Yang, K., Faustinella, F., Xue, J. J., Whitson, J., Kampfl, A., Mu, X. S., Zhao, X., Taglialatela, G., Perez-Polo, J. R., Clifton, G., & Hayes, R. L. (1994). Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer. Neuroscience Letters, 182(2), 291-294. https://doi.org/10.1016/0304-3940(94)90819-2

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title = "Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer",

abstract = "We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.",

keywords = "Gene transfer, Liposome, NGF, Septo-hippocampal cell culture",

author = "K. Yang and F. Faustinella and Xue, {J. J.} and J. Whitson and A. Kampfl and Mu, {X. S.} and X. Zhao and G. Taglialatela and Perez-Polo, {J. R.} and G. Clifton and Hayes, {R. L.}",

note = "Funding Information: We thank Shannon Garcia for critical editing of this manuscript. This work was supported by Grants P01 NS3199801 and NS21458 from the National Institutes of Health. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1994",

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doi = "10.1016/0304-3940(94)90819-2",

language = "English (US)",

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pages = "291--294",

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T1 - Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer

AU - Yang, K.

AU - Faustinella, F.

AU - Xue, J. J.

AU - Whitson, J.

AU - Kampfl, A.

AU - Mu, X. S.

AU - Zhao, X.

AU - Taglialatela, G.

AU - Perez-Polo, J. R.

AU - Clifton, G.

AU - Hayes, R. L.

N1 - Funding Information: We thank Shannon Garcia for critical editing of this manuscript. This work was supported by Grants P01 NS3199801 and NS21458 from the National Institutes of Health. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1994/12/5

Y1 - 1994/12/5

N2 - We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.

AB - We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.

KW - Gene transfer

KW - Liposome

KW - NGF

KW - Septo-hippocampal cell culture

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Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer

Abstract

Keywords

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