Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer

K. Yang, F. Faustinella, J. J. Xue, J. Whitson, A. Kampfl, X. S. Mu, X. Zhao, Giulio Taglialatela, J. R. Perez-Polo, G. Clifton, R. L. Hayes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.

Original languageEnglish (US)
Pages (from-to)291-294
Number of pages4
JournalNeuroscience Letters
Volume182
Issue number2
DOIs
StatePublished - Dec 5 1994

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Nerve Growth Factor
Liposomes
Cell Culture Techniques
Genes
Transfection
Biological Assay
Culture Media
Central Nervous System
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Gene transfer
  • Liposome
  • NGF
  • Septo-hippocampal cell culture

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer. / Yang, K.; Faustinella, F.; Xue, J. J.; Whitson, J.; Kampfl, A.; Mu, X. S.; Zhao, X.; Taglialatela, Giulio; Perez-Polo, J. R.; Clifton, G.; Hayes, R. L.

In: Neuroscience Letters, Vol. 182, No. 2, 05.12.1994, p. 291-294.

Research output: Contribution to journalArticle

Yang, K, Faustinella, F, Xue, JJ, Whitson, J, Kampfl, A, Mu, XS, Zhao, X, Taglialatela, G, Perez-Polo, JR, Clifton, G & Hayes, RL 1994, 'Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer', Neuroscience Letters, vol. 182, no. 2, pp. 291-294. https://doi.org/10.1016/0304-3940(94)90819-2
Yang, K. ; Faustinella, F. ; Xue, J. J. ; Whitson, J. ; Kampfl, A. ; Mu, X. S. ; Zhao, X. ; Taglialatela, Giulio ; Perez-Polo, J. R. ; Clifton, G. ; Hayes, R. L. / Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer. In: Neuroscience Letters. 1994 ; Vol. 182, No. 2. pp. 291-294.
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