Abstract
Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (γH2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 352-359 |
| Number of pages | 8 |
| Journal | Gene |
| Volume | 527 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 15 2013 |
| Externally published | Yes |
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Keywords
- γH2AX
- KAT/TIP60
- Meiosis
- SLX2
- SYCE2
ASJC Scopus subject areas
- Genetics
Cite this
SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60. / Shi, Yu Qiang; Zhuang, Xin Jie; Xu, Bo; Hua, Juan; Liao, Shang Ying; Shi, Qinghua; Cooke, Howard J.; Han, Chunsheng.
In: Gene, Vol. 527, No. 1, 15.09.2013, p. 352-359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60
AU - Shi, Yu Qiang
AU - Zhuang, Xin Jie
AU - Xu, Bo
AU - Hua, Juan
AU - Liao, Shang Ying
AU - Shi, Qinghua
AU - Cooke, Howard J.
AU - Han, Chunsheng
PY - 2013/9/15
Y1 - 2013/9/15
N2 - Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (γH2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis.
AB - Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (γH2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis.
KW - γH2AX
KW - KAT/TIP60
KW - Meiosis
KW - SLX2
KW - SYCE2
UR - http://www.scopus.com/inward/record.url?scp=84881253422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881253422&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2013.06.033
DO - 10.1016/j.gene.2013.06.033
M3 - Article
C2 - 23810942
AN - SCOPUS:84881253422
VL - 527
SP - 352
EP - 359
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -