SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60

Yu Qiang Shi, Xin Jie Zhuang, Bo Xu, Juan Hua, Shang Ying Liao, Qinghua Shi, Howard J. Cooke, Chunsheng Han

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (γH2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis.

Original languageEnglish (US)
Pages (from-to)352-359
Number of pages8
JournalGene
Volume527
Issue number1
DOIs
StatePublished - Sep 15 2013
Externally publishedYes

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Synaptonemal Complex
Histone Acetyltransferases
Germ Cells
Pachytene Stage
Meiotic Prophase I
Meiosis
Proteins
Spermatocytes
Nucleosomes
Y Chromosome
Haploidy
X Chromosome
Diploidy
Histones
Genetic Recombination
DNA Damage
Yeasts
Maintenance
Lymphocytes
Gene Expression

Keywords

  • γH2AX
  • KAT/TIP60
  • Meiosis
  • SLX2
  • SYCE2

ASJC Scopus subject areas

  • Genetics

Cite this

SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60. / Shi, Yu Qiang; Zhuang, Xin Jie; Xu, Bo; Hua, Juan; Liao, Shang Ying; Shi, Qinghua; Cooke, Howard J.; Han, Chunsheng.

In: Gene, Vol. 527, No. 1, 15.09.2013, p. 352-359.

Research output: Contribution to journalArticle

Shi, Yu Qiang ; Zhuang, Xin Jie ; Xu, Bo ; Hua, Juan ; Liao, Shang Ying ; Shi, Qinghua ; Cooke, Howard J. ; Han, Chunsheng. / SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60. In: Gene. 2013 ; Vol. 527, No. 1. pp. 352-359.
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