Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

Ramon Eritja, Bruce E. Kaplan, Dhananjaya Mhaskar, Lawrence Sowers, John Petruska, Myron F. Goodman

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

Original languageEnglish (US)
Pages (from-to)5869-5884
Number of pages16
JournalNucleic Acids Research
Volume14
Issue number14
DOIs
StatePublished - Jul 11 1986
Externally publishedYes

Fingerprint

2-Aminopurine
Oligomers
Base Pairing
DNA
Synthesis
DNA-Directed DNA Polymerase
Relative Stability
APT
Analogue

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine. / Eritja, Ramon; Kaplan, Bruce E.; Mhaskar, Dhananjaya; Sowers, Lawrence; Petruska, John; Goodman, Myron F.

In: Nucleic Acids Research, Vol. 14, No. 14, 11.07.1986, p. 5869-5884.

Research output: Contribution to journalArticle

Eritja, Ramon ; Kaplan, Bruce E. ; Mhaskar, Dhananjaya ; Sowers, Lawrence ; Petruska, John ; Goodman, Myron F. / Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine. In: Nucleic Acids Research. 1986 ; Vol. 14, No. 14. pp. 5869-5884.
@article{a29012e49fb44bd794bc8c39fcc38bbb,
title = "Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine",
abstract = "DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.",
author = "Ramon Eritja and Kaplan, {Bruce E.} and Dhananjaya Mhaskar and Lawrence Sowers and John Petruska and Goodman, {Myron F.}",
year = "1986",
month = "7",
day = "11",
doi = "10.1093/nar/14.14.5869",
language = "English (US)",
volume = "14",
pages = "5869--5884",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "14",

}

TY - JOUR

T1 - Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

AU - Eritja, Ramon

AU - Kaplan, Bruce E.

AU - Mhaskar, Dhananjaya

AU - Sowers, Lawrence

AU - Petruska, John

AU - Goodman, Myron F.

PY - 1986/7/11

Y1 - 1986/7/11

N2 - DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

AB - DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

UR - http://www.scopus.com/inward/record.url?scp=0023056882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023056882&partnerID=8YFLogxK

U2 - 10.1093/nar/14.14.5869

DO - 10.1093/nar/14.14.5869

M3 - Article

VL - 14

SP - 5869

EP - 5884

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 14

ER -