Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

Ramon Eritja; Bruce E. Kaplan; Dhananjaya Mhaskar; Lawrence C. Sowers; John Petruska; Myron F. Goodman

doi:10.1093/nar/14.14.5869

Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

Ramon Eritja, Bruce E. Kaplan, Dhananjaya Mhaskar, Lawrence C. Sowers, John Petruska, Myron F. Goodman

Research output: Contribution to journal › Article › peer-review

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Abstract

DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

Original language	English (US)
Pages (from-to)	5869-5884
Number of pages	16
Journal	Nucleic acids research
Volume	14
Issue number	14
DOIs	https://doi.org/10.1093/nar/14.14.5869
State	Published - Jul 11 1986
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{a29012e49fb44bd794bc8c39fcc38bbb,

title = "Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine",

abstract = "DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.",

author = "Ramon Eritja and Kaplan, {Bruce E.} and Dhananjaya Mhaskar and Sowers, {Lawrence C.} and John Petruska and Goodman, {Myron F.}",

note = "Funding Information: R. E. is recipient of a postdoctoral fellowship from the Fundacion JUAN MARCH, Spain. He thank T. Huang forhishelp during the DNA sequencing andH. Kim forassistance inobtaining NMRspectra. Support forthis work was provided by grants froo the National Institutes of Health GM21422 and GM33863.",

year = "1986",

month = jul,

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doi = "10.1093/nar/14.14.5869",

language = "English (US)",

volume = "14",

pages = "5869--5884",

journal = "Nucleic acids research",

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T1 - Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

AU - Eritja, Ramon

AU - Kaplan, Bruce E.

AU - Mhaskar, Dhananjaya

AU - Sowers, Lawrence C.

AU - Petruska, John

AU - Goodman, Myron F.

N1 - Funding Information: R. E. is recipient of a postdoctoral fellowship from the Fundacion JUAN MARCH, Spain. He thank T. Huang forhishelp during the DNA sequencing andH. Kim forassistance inobtaining NMRspectra. Support forthis work was provided by grants froo the National Institutes of Health GM21422 and GM33863.

PY - 1986/7/11

Y1 - 1986/7/11

N2 - DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

AB - DNA heptamers containing the mutagenic base analogue 2- aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330nm. Base pairs are ranked in order of decreasing stability: AP·T > AP·A> AP·C > AP·G. It is of interest that AP·A is more stable than AP·C even though DNA polymerase strongly favors the formation of AP·C over AP·A base pairs. Comparisons of nelting profiles at 330nm and 275nm indicate that AP·T, AP·A, and AP·C base pairs are annealed in heptamer duplexes and melt 2-3° prior to surrounding base pairs, whereas AP·G appears not to be annealed.

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JO - Nucleic acids research

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Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine

Abstract

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