Synthesis and utilization of 13C(8)-enriched purines

Christopher J. LaFrancois, June Fujimoto, Lawrence Sowers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A new and more efficient method is presented for the synthesis of 13C(8)-enriched adenine. In addition, we present for the first time the synthesis of 13C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of 13C-enrichment in heteronuclear isotope- edited NMR spectra.

Original languageEnglish (US)
Pages (from-to)23-37
Number of pages15
JournalNucleosides and Nucleotides
Volume18
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Purines
Adenine
2-Aminopurine
Ribonucleosides
Nucleosides
Oligonucleotides
Isotopes
Nuclear magnetic resonance
purine

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Synthesis and utilization of 13C(8)-enriched purines. / LaFrancois, Christopher J.; Fujimoto, June; Sowers, Lawrence.

In: Nucleosides and Nucleotides, Vol. 18, No. 1, 1999, p. 23-37.

Research output: Contribution to journalArticle

LaFrancois, CJ, Fujimoto, J & Sowers, L 1999, 'Synthesis and utilization of 13C(8)-enriched purines', Nucleosides and Nucleotides, vol. 18, no. 1, pp. 23-37.
LaFrancois, Christopher J. ; Fujimoto, June ; Sowers, Lawrence. / Synthesis and utilization of 13C(8)-enriched purines. In: Nucleosides and Nucleotides. 1999 ; Vol. 18, No. 1. pp. 23-37.
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