Synthesis of protein in intestinal cells exposed to cholera toxin

Johnny Peterson, W. D. Berg, D. H. Coppenhaver

Research output: Contribution to journalArticle

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Abstract

The mechanism by which cyclic adenosine monophosphate (AMP), formed by intestinal epithelial cells in response to cholera toxin, ultimately results in alterations in water and electrolyte transport is poorly understood. Several studies have indicated that inhibitors of transcription or translation block much of the transport of ions and water in the intestine and edema formation in tissue elecited by cholera toxin. Data presented in this study confirmed the inhibitory effects of cycloheximide on cholera toxin-induced fluid accumulation in the rabbit intestinal loop model. Neither cycloheximide nor actinomycin D altered the amount of cylic AMP that accumulated in intestinal cells and Chinese hamster ovary cells exposed to cholera toxin. An increase in [3H]leucine incorporation was readily demonstrable in intestinal epithelial cells from rabbits challenged with Vibrio cholerae. Similarly, intestinal epithelial cells incubated with cholera toxin for 4 hr synthesized substantially more protein than controls as determined by relative incorporation of [35S]methionine. Most of the new protein synthesized in response to cholera toxin was membrane associated and of high molecular weight. The possible significance of the toxin-induced protein relative to cholera pathogenesis was discussed.

Original languageEnglish (US)
Pages (from-to)174-182
Number of pages9
JournalProceedings of the Society for Experimental Biology and Medicine
Volume186
Issue number2
StatePublished - 1987

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Cholera Toxin
Proteins
Epithelial Cells
Cycloheximide
Rabbits
Vibrio cholerae
Water
Cholera
Ion Transport
Dactinomycin
Adenosine Monophosphate
Transcription
Cricetulus
Leucine
Methionine
Cyclic AMP
Electrolytes
Intestines
Ovary
Edema

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Synthesis of protein in intestinal cells exposed to cholera toxin. / Peterson, Johnny; Berg, W. D.; Coppenhaver, D. H.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 186, No. 2, 1987, p. 174-182.

Research output: Contribution to journalArticle

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