Synthesis of Seoul virus RNA and structural proteins in cultured cells

Hiroaki Kariwa, H. Tanabe, T. Mizutani, Y. Kon, K. Lokugamage, N. Lokugamage, M. A. Iwasa, T. Hagiya, K. Araki, K. Yoshimatsu, J. Arikawa, I. Takashima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). The virion has a tripartite (S, M, and L) negative-stranded RNA genome, which is characteristic of the family Bunyaviridae. However, the molecular basis of virus replication is not well known. We established a Northern blot hybridization (NB) procedure using digoxygenin-labeled RNA probes, to quantitate the hantaviral plus- and minus-strand RNAs separately. Virus RNA replication was analyzed in infected Vero E6 cells. When the Vero E6 cells were infected with Seoul virus strain KI-83-262 (KI) at m.o.i. = 0.25, the plus-strand RNA was detected within 1 h post-infection (hpi), and the minus-strand RNA was detected subsequently. Using laser confocal microscopy, the nucleocapsid protein (NP) was detected within 2 hpi, and accumulated as scattered granules in the cytoplasm until 24 hpi. In contrast, the G2 protein first appeared at 8 hpi, was immediately transported to the Golgi, and accumulated in the Golgi until 24 hpi. Infectious virus particles were released into the medium at 24 h hpi. These findings indicate that hantavirus RNA replication starts with the appearance of NP at 2 hpi, glycoproteins then accumulate gradually in the Golgi, and virion formation is initiated once the viral RNAs and proteins have accumulated.

Original languageEnglish (US)
Pages (from-to)1671-1685
Number of pages15
JournalArchives of Virology
Volume148
Issue number9
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Seoul virus
Viral Structural Proteins
Cultured Cells
RNA
Infection
Virion
Hantavirus
Nucleocapsid Proteins
Vero Cells
Virus Replication
Confocal Microscopy
Bunyaviridae
Hemorrhagic Fever with Renal Syndrome
RNA Probes
Viral RNA
Viral Proteins
Northern Blotting
Glycoproteins
Cytoplasm
Genome

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Kariwa, H., Tanabe, H., Mizutani, T., Kon, Y., Lokugamage, K., Lokugamage, N., ... Takashima, I. (2003). Synthesis of Seoul virus RNA and structural proteins in cultured cells. Archives of Virology, 148(9), 1671-1685. https://doi.org/10.1007/s00705-003-0141-6

Synthesis of Seoul virus RNA and structural proteins in cultured cells. / Kariwa, Hiroaki; Tanabe, H.; Mizutani, T.; Kon, Y.; Lokugamage, K.; Lokugamage, N.; Iwasa, M. A.; Hagiya, T.; Araki, K.; Yoshimatsu, K.; Arikawa, J.; Takashima, I.

In: Archives of Virology, Vol. 148, No. 9, 01.09.2003, p. 1671-1685.

Research output: Contribution to journalArticle

Kariwa, H, Tanabe, H, Mizutani, T, Kon, Y, Lokugamage, K, Lokugamage, N, Iwasa, MA, Hagiya, T, Araki, K, Yoshimatsu, K, Arikawa, J & Takashima, I 2003, 'Synthesis of Seoul virus RNA and structural proteins in cultured cells', Archives of Virology, vol. 148, no. 9, pp. 1671-1685. https://doi.org/10.1007/s00705-003-0141-6
Kariwa H, Tanabe H, Mizutani T, Kon Y, Lokugamage K, Lokugamage N et al. Synthesis of Seoul virus RNA and structural proteins in cultured cells. Archives of Virology. 2003 Sep 1;148(9):1671-1685. https://doi.org/10.1007/s00705-003-0141-6
Kariwa, Hiroaki ; Tanabe, H. ; Mizutani, T. ; Kon, Y. ; Lokugamage, K. ; Lokugamage, N. ; Iwasa, M. A. ; Hagiya, T. ; Araki, K. ; Yoshimatsu, K. ; Arikawa, J. ; Takashima, I. / Synthesis of Seoul virus RNA and structural proteins in cultured cells. In: Archives of Virology. 2003 ; Vol. 148, No. 9. pp. 1671-1685.
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