Synthesis, quantification, characterization, and signaling properties of glutathionyl conjugates of enals.

Sanjay Srivastava, Kota Ramana, Aruni Bhatnagar, Satish Srivastava

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Oxidation of lipids generates large quantities of highly reactive alpha,beta-unsaturated aldehydes (enals). Enals and their protein adducts accumulate in the tissues of several pathologies. In vitro, low concentrations of enals such as HNE (4-hydroxy trans-2-nonenal) affect cell signaling whereas high concentrations of enals are cytotoxic. Direct conjugation of the C2-C3 double bond of enals with the sulfhydryl group of GSH is a major route for the metabolism and detoxification of enals. Recently, we found that glutathionyl conjugate of HNE (GS-HNE) enhances the peritoneal leukocyte infiltration and stimulates the formation of proinflammatory lipid mediators. Moreover, the reduced form of the glutathione conjugate of HNE (GS-DHN) elicits strong mitogenic signaling in smooth muscle cells. In this chapter we discuss the methods to study the metabolism of enals and the redox signaling properties of glutathionyl conjugates of HNE. Copyright (c) 2010 Elsevier Inc. All rights reserved.

Original languageEnglish (US)
Pages (from-to)297-313
Number of pages17
JournalMethods in Enzymology
Volume474
StatePublished - 2010
Externally publishedYes

Fingerprint

Metabolism
Cell signaling
Lipids
Detoxification
Pathology
Infiltration
Aldehydes
Oxidation-Reduction
Smooth Muscle Myocytes
Glutathione
Muscle
Leukocytes
Cells
Tissue
Oxidation
Proteins
glutathionyl 4-hydroxy-2-nonenal conjugate
2-nonenal
In Vitro Techniques
glutathionyl-1,4-dihydroxynonane

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Synthesis, quantification, characterization, and signaling properties of glutathionyl conjugates of enals. / Srivastava, Sanjay; Ramana, Kota; Bhatnagar, Aruni; Srivastava, Satish.

In: Methods in Enzymology, Vol. 474, 2010, p. 297-313.

Research output: Contribution to journalArticle

Srivastava, Sanjay ; Ramana, Kota ; Bhatnagar, Aruni ; Srivastava, Satish. / Synthesis, quantification, characterization, and signaling properties of glutathionyl conjugates of enals. In: Methods in Enzymology. 2010 ; Vol. 474. pp. 297-313.
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