System and methods for wide-field quantitative fluorescence imaging during neurosurgery

Pablo A. Valdes, Valerie L. Jacobs, Brian C. Wilson, Frederic Leblond, David W. Roberts, Keith D. Paulsen

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


We report an accurate, precise and sensitive method and system for quantitative fluorescence image-guided neurosurgery. With a low-noise, high-dynamic-range CMOS array, we performrapid (integration times as low as 50 ms per wavelength) hyperspectral fluorescence and diffuse reflectance detection and apply a correction algorithm to compensate for the distorting effects of tissue absorption and scattering. Using this approach, we generated quantitative wide-field images of fluorescence in tissue-simulating phantoms for the fluorophore PpIX, having concentrations and optical absorption and scattering variations over clinically relevant ranges. The imaging system was tested in a rodent model of glioma, detecting quantitative levels down to 20 ng/ml. The resulting performance is a significant advance on existing wide-field quantitative imaging techniques, and provides performance comparable to a pointspectroscopy probe that has previously demonstrated significant potential for improved detection of malignant brain tumors during surgical resection.

Original languageEnglish (US)
Pages (from-to)2786-2788
Number of pages3
JournalOptics Letters
Issue number15
StatePublished - Aug 1 2013
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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