Systematic analysis of cell-type differences in the epithelial secretome reveals insights into the pathogenesis of respiratory syncytial virus-induced lower respiratory tract infections

Yingxin Zhao, Mohammad Jamaluddin, Yueqing Zhang, Hong Sun, Teodora Ivanciuc, Roberto Garofalo, Allan R. Brasier

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lower respiratory tract infections from respiratory syncytial virus (RSV) are due, in part, to secreted signals from lower airway cells that modify the immune response and trigger airway remodeling. To understand this process, we applied an unbiased quantitative proteomics analysis of the RSV-induced epithelial secretory response in cells representative of the trachea versus small airway bronchiolar cells. A workflow was established using telomerase-immortalized human epithelial cells that revealed highly reproducible cell type-specific differences in secreted proteins and nanoparticles (exosomes). Approximately one third of secretome proteins are exosomal; the remainder are from lysosomal and vacuolar compartments. We applied this workflow to three independently derived primary human cultures from trachea versus bronchioles. A total of 577 differentially expressed proteins from control supernatants and 966 differentially expressed proteins from RSV-infected cell supernatants were identified at a 1% false discovery rate. Fifteen proteins unique to RSV-infected primary human cultures from trachea were regulated by epithelialspecific ets homologous factor. A total of 106 proteins unique to RSV-infected human small airway epithelial cells was regulated by the transcription factor NF-kB. In this latter group, we validated the differential expression of CCL20/macrophage-inducible protein 3a, thymic stromal lymphopoietin, and CCL3-like 1 because of their roles in Th2 polarization. CCL20/macrophageinducible protein 3a was the most active mucin-inducing factor in the RSV-infected human small airway epithelial cell secretome and was differentially expressed in smaller airways in a mouse model of RSV infection. These studies provide insights into the complexity of innate responses and regional differences in the epithelial secretome participating in RSV lower respiratory tract infection-induced airway remodeling.

Original languageEnglish (US)
Pages (from-to)3345-3364
Number of pages20
JournalJournal of Immunology
Volume198
Issue number8
DOIs
StatePublished - Apr 15 2017

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Respiratory Syncytial Viruses
Respiratory Tract Infections
Proteins
Trachea
Human respiratory syncytial virus
Airway Remodeling
Workflow
Epithelial Cells
Exosomes
Bronchioles
Respiratory Syncytial Virus Infections
NF-kappa B
Telomerase
Mucins
Proteomics
Nanoparticles
Macrophages

ASJC Scopus subject areas

  • Immunology

Cite this

Systematic analysis of cell-type differences in the epithelial secretome reveals insights into the pathogenesis of respiratory syncytial virus-induced lower respiratory tract infections. / Zhao, Yingxin; Jamaluddin, Mohammad; Zhang, Yueqing; Sun, Hong; Ivanciuc, Teodora; Garofalo, Roberto; Brasier, Allan R.

In: Journal of Immunology, Vol. 198, No. 8, 15.04.2017, p. 3345-3364.

Research output: Contribution to journalArticle

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