Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica after intrapulmonary inoculation.

Jere McBride, R. E. Corstvet, D. B. Paulsen, J. R. McClure, F. M. Enright

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Abstract

Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica were evaluated by measuring immunoglobulin production in blood for 9 days and in pulmonary lavage fluid for 7 days after intrapulmonary inoculation. Clinical signs, pulmonary lesions, pulmonary and systemic inflammatory response, and amount of antigen in lavage fluid were used to evaluate the response of calves to challenge with P haemolytica. The pulmonary response consisted of production of IgG, IgE, and IgM antibodies to P haemolytica antigens and a 17- to 68-fold increase of cells in lavage fluid 8 hours after inoculation, with a gradual decrease toward normal. Antibodies of the IgM isotype to P haemolytica were demonstrated as early as 8 hours through 7 days after inoculation in 3 of 3 calves. Of the anti-P haemolytica isotypes, IgM was found in the highest concentration. In all of the inoculated calves, IgE was found 1 to 2 days after inoculation, and IgG was found in 2 of 3 inoculated calves from day 1 through 7 after inoculation. Detection of IgG correlated with smaller pulmonary lesions. Immunoglobulin A was not detected in lavage fluid. Serum was evaluated for IgG and IgM antibody response to P haemolytica. Specific IgM was detectable 5 days after inoculation, and IgG was detectable 7 days after inoculation. Pasteurella haemolytica antigens were not detected in serum or plasma. A transient increase in neutrophil count was found 8 hours after inoculation, with return to baseline values by 24 hours after inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)1889-1894
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume53
Issue number10
StatePublished - Oct 1992
Externally publishedYes

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Mannheimia haemolytica
Antibody Formation
Immunoglobulin M
Immunoglobulin G
lungs
calves
Therapeutic Irrigation
Lung
antibodies
Antigens
Immunoglobulin E
blood serum
antigens
lesions (animal)
Antibodies
Bronchoalveolar Lavage Fluid
immunoglobulin A
Serum
Immunoglobulin A
Immunoglobulins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica after intrapulmonary inoculation. / McBride, Jere; Corstvet, R. E.; Paulsen, D. B.; McClure, J. R.; Enright, F. M.

In: American Journal of Veterinary Research, Vol. 53, No. 10, 10.1992, p. 1889-1894.

Research output: Contribution to journalArticle

McBride, Jere ; Corstvet, R. E. ; Paulsen, D. B. ; McClure, J. R. ; Enright, F. M. / Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica after intrapulmonary inoculation. In: American Journal of Veterinary Research. 1992 ; Vol. 53, No. 10. pp. 1889-1894.
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