T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia

F. T. Silveira, J. M. Blackwell, E. A. Ishikawa, R. Braga, J. J. Shaw, R. J. Quinnell, Lynn Soong, P. Kima, D. McMahon-Pratt, G. F. Black, M. A. Shaw

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Amazonian localized cutaneous leishmaniasis (LCL) is caused by parasites of the subgenera Leishmania and Viannia. Respectively, these parasites may cause diffuse cutaneous leishmaniasis (DCL) and mucocutaneous leishmaniasis (MCL). This, together with differing skin test responses, suggests some species-specificity in cell mediated immunity. In this study, T cell responses (proliferative and interferon-γ) to crude and defined antigens were examined in paired samples pre and post chemotherapy. Untreated L. (L.) amazonensis LCL patients showed lower responses to crude leishmanial antigens than the L. (V.) spp. group. L. (V.) braziliensis antigen was a more potent stimulator of T cell responses than L. (L.) amazonensis antigen in all patient groups. Few positive responses were seen to the L. (L.) amazonensis glycoprotein GP46. A substantial proportion of LCL patients did respond to the L. (L.) pifanoi amastigote antigens A2, and the surface membrane glycoprotein P8. DCL patients were poor responders to all leishmanial antigens, except GP46. In contrast, MCL patients were good responders to all antigens except GP46 and A2. A significant rise in the response to P8 and A2 antigen was seen post treatment across all LCL and MCL patients, indicating that these antigens might provide suitable vaccine candidates.

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalParasite Immunology
Volume20
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

Leishmania
Brazil
Cutaneous Leishmaniasis
Mucocutaneous Leishmaniasis
T-Lymphocytes
Antigens
Diffuse Cutaneous Leishmaniasis
varespladib methyl
Membrane Glycoproteins
Parasites
Species Specificity
Skin Tests
Cellular Immunity
Interferons
Glycoproteins
Vaccines
Drug Therapy

Keywords

  • Cutaneous leishmaniasis
  • T cell therapy

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia. / Silveira, F. T.; Blackwell, J. M.; Ishikawa, E. A.; Braga, R.; Shaw, J. J.; Quinnell, R. J.; Soong, Lynn; Kima, P.; McMahon-Pratt, D.; Black, G. F.; Shaw, M. A.

In: Parasite Immunology, Vol. 20, No. 1, 1998, p. 19-26.

Research output: Contribution to journalArticle

Silveira, FT, Blackwell, JM, Ishikawa, EA, Braga, R, Shaw, JJ, Quinnell, RJ, Soong, L, Kima, P, McMahon-Pratt, D, Black, GF & Shaw, MA 1998, 'T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia', Parasite Immunology, vol. 20, no. 1, pp. 19-26.
Silveira, F. T. ; Blackwell, J. M. ; Ishikawa, E. A. ; Braga, R. ; Shaw, J. J. ; Quinnell, R. J. ; Soong, Lynn ; Kima, P. ; McMahon-Pratt, D. ; Black, G. F. ; Shaw, M. A. / T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia. In: Parasite Immunology. 1998 ; Vol. 20, No. 1. pp. 19-26.
@article{06af136a656241f8a7664d72920bf835,
title = "T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia",
abstract = "Amazonian localized cutaneous leishmaniasis (LCL) is caused by parasites of the subgenera Leishmania and Viannia. Respectively, these parasites may cause diffuse cutaneous leishmaniasis (DCL) and mucocutaneous leishmaniasis (MCL). This, together with differing skin test responses, suggests some species-specificity in cell mediated immunity. In this study, T cell responses (proliferative and interferon-γ) to crude and defined antigens were examined in paired samples pre and post chemotherapy. Untreated L. (L.) amazonensis LCL patients showed lower responses to crude leishmanial antigens than the L. (V.) spp. group. L. (V.) braziliensis antigen was a more potent stimulator of T cell responses than L. (L.) amazonensis antigen in all patient groups. Few positive responses were seen to the L. (L.) amazonensis glycoprotein GP46. A substantial proportion of LCL patients did respond to the L. (L.) pifanoi amastigote antigens A2, and the surface membrane glycoprotein P8. DCL patients were poor responders to all leishmanial antigens, except GP46. In contrast, MCL patients were good responders to all antigens except GP46 and A2. A significant rise in the response to P8 and A2 antigen was seen post treatment across all LCL and MCL patients, indicating that these antigens might provide suitable vaccine candidates.",
keywords = "Cutaneous leishmaniasis, T cell therapy",
author = "Silveira, {F. T.} and Blackwell, {J. M.} and Ishikawa, {E. A.} and R. Braga and Shaw, {J. J.} and Quinnell, {R. J.} and Lynn Soong and P. Kima and D. McMahon-Pratt and Black, {G. F.} and Shaw, {M. A.}",
year = "1998",
language = "English (US)",
volume = "20",
pages = "19--26",
journal = "Parasite Immunology",
issn = "0141-9838",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - T cell responses to crude and defined leishmanial antigens in patients from the lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and Viannia

AU - Silveira, F. T.

AU - Blackwell, J. M.

AU - Ishikawa, E. A.

AU - Braga, R.

AU - Shaw, J. J.

AU - Quinnell, R. J.

AU - Soong, Lynn

AU - Kima, P.

AU - McMahon-Pratt, D.

AU - Black, G. F.

AU - Shaw, M. A.

PY - 1998

Y1 - 1998

N2 - Amazonian localized cutaneous leishmaniasis (LCL) is caused by parasites of the subgenera Leishmania and Viannia. Respectively, these parasites may cause diffuse cutaneous leishmaniasis (DCL) and mucocutaneous leishmaniasis (MCL). This, together with differing skin test responses, suggests some species-specificity in cell mediated immunity. In this study, T cell responses (proliferative and interferon-γ) to crude and defined antigens were examined in paired samples pre and post chemotherapy. Untreated L. (L.) amazonensis LCL patients showed lower responses to crude leishmanial antigens than the L. (V.) spp. group. L. (V.) braziliensis antigen was a more potent stimulator of T cell responses than L. (L.) amazonensis antigen in all patient groups. Few positive responses were seen to the L. (L.) amazonensis glycoprotein GP46. A substantial proportion of LCL patients did respond to the L. (L.) pifanoi amastigote antigens A2, and the surface membrane glycoprotein P8. DCL patients were poor responders to all leishmanial antigens, except GP46. In contrast, MCL patients were good responders to all antigens except GP46 and A2. A significant rise in the response to P8 and A2 antigen was seen post treatment across all LCL and MCL patients, indicating that these antigens might provide suitable vaccine candidates.

AB - Amazonian localized cutaneous leishmaniasis (LCL) is caused by parasites of the subgenera Leishmania and Viannia. Respectively, these parasites may cause diffuse cutaneous leishmaniasis (DCL) and mucocutaneous leishmaniasis (MCL). This, together with differing skin test responses, suggests some species-specificity in cell mediated immunity. In this study, T cell responses (proliferative and interferon-γ) to crude and defined antigens were examined in paired samples pre and post chemotherapy. Untreated L. (L.) amazonensis LCL patients showed lower responses to crude leishmanial antigens than the L. (V.) spp. group. L. (V.) braziliensis antigen was a more potent stimulator of T cell responses than L. (L.) amazonensis antigen in all patient groups. Few positive responses were seen to the L. (L.) amazonensis glycoprotein GP46. A substantial proportion of LCL patients did respond to the L. (L.) pifanoi amastigote antigens A2, and the surface membrane glycoprotein P8. DCL patients were poor responders to all leishmanial antigens, except GP46. In contrast, MCL patients were good responders to all antigens except GP46 and A2. A significant rise in the response to P8 and A2 antigen was seen post treatment across all LCL and MCL patients, indicating that these antigens might provide suitable vaccine candidates.

KW - Cutaneous leishmaniasis

KW - T cell therapy

UR - http://www.scopus.com/inward/record.url?scp=15644370348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15644370348&partnerID=8YFLogxK

M3 - Article

C2 - 9491414

AN - SCOPUS:15644370348

VL - 20

SP - 19

EP - 26

JO - Parasite Immunology

JF - Parasite Immunology

SN - 0141-9838

IS - 1

ER -