T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus

John T. Roehrig, Peggy A. Risi, John R. Brubaker, Ann R. Hunt, Barry J. Beaty, Dennis W. Trent, James H. Mathews

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Abstract

To identify T-helper (Th)-cell epitopes, we analyzed 25 synthetic peptides, which included most of the 495-amino-acid sequence of the envelope (E)-glycoprotein of dengue 2 virus. The peptides were analyzed in three mouse strains, BALB/c (H-2d), C57BL/6 (H-2b), and outbred NIH-Swiss, for their ability to elicit antibody or prime the Th-cell compartment following two inoculations in Freund's incomplete adjuvant. Sixteen peptides were able to elicit an antipeptide antibody response in one or more mouse strain. Eleven antipeptide serum pools were able to bind to virus in ELISA. Fifteen peptides primed one or more haplotype for an in vitro antipeptide Th-cell response as measured by blastogenesis. Th-cell activation was generally confirmed by measurable in vitro production of intedeukin (IL)-2/IL-4. Nine peptides that were positive for in vitro blastogenesis, 1-2, 35, 4-6, 79, 142, 208, 06, 16, and 17, elicited virus-reactive Th-cells in vitro in H-2d mice. Two of these peptides (4-6 and 17) were able to prime virus-reactive Th-cells in H-2b mice. Nine peptides primed outbred mice in vivo for an antiviral antibody response significantly greater than that seen in animals primed with an irrelevant peptide. These results correlate with, and expand on, our previous observations based on a smaller set of synthetic peptides derived from the E-glycoprotein of Murray Valley encephalitis virus and suggest that synthetic peptides can function as E-glycoprotein Th-cell epitopes. The similarity of results between two distantly related flaviviruses suggests that E-glycoprotein Th-cell epitopes are consistent in location and activity.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalVirology
Volume198
Issue number1
DOIs
StatePublished - Jan 1994

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Jamaica
T-Lymphocyte Epitopes
Peptides
Helper-Inducer T-Lymphocytes
Lymphocyte Activation
Viruses
Antibody Formation
Murray Valley encephalitis virus
Glycoproteins
Dengue virus type 2 E-glycoprotein
Inbred C57BL Mouse
Interleukin-4
Haplotypes
Interleukin-2
Antiviral Agents
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Roehrig, J. T., Risi, P. A., Brubaker, J. R., Hunt, A. R., Beaty, B. J., Trent, D. W., & Mathews, J. H. (1994). T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus. Virology, 198(1), 31-38. https://doi.org/10.1006/viro.1994.1005

T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus. / Roehrig, John T.; Risi, Peggy A.; Brubaker, John R.; Hunt, Ann R.; Beaty, Barry J.; Trent, Dennis W.; Mathews, James H.

In: Virology, Vol. 198, No. 1, 01.1994, p. 31-38.

Research output: Contribution to journalArticle

Roehrig, JT, Risi, PA, Brubaker, JR, Hunt, AR, Beaty, BJ, Trent, DW & Mathews, JH 1994, 'T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus', Virology, vol. 198, no. 1, pp. 31-38. https://doi.org/10.1006/viro.1994.1005
Roehrig JT, Risi PA, Brubaker JR, Hunt AR, Beaty BJ, Trent DW et al. T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus. Virology. 1994 Jan;198(1):31-38. https://doi.org/10.1006/viro.1994.1005
Roehrig, John T. ; Risi, Peggy A. ; Brubaker, John R. ; Hunt, Ann R. ; Beaty, Barry J. ; Trent, Dennis W. ; Mathews, James H. / T-Helper Cell Epitopes on the E-Glycoprotein of Dengue 2 Jamaica Virus. In: Virology. 1994 ; Vol. 198, No. 1. pp. 31-38.
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