Taenia saginata oncosphere excretory/secretory peptidases

A. Clinton White, Salman Baig, Prema Robinson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To identify oncosphere excretory/secretory peptidases, Taenia saginata adult worms were collected from 3 patients. Eggs were hatched and activated in vitro and oncospheres cultured in vitro. The culture medium from the oncospheres was assayed with peptide substrates coupled to 7-amino-4- trifluoromethyl coumarin (AFC), and free AFC was detected fluorometrically. The endopeptidase substrates Z-Phe-Arg-AFC and Z-Arg-AFC as well as the aminopeptidase substrate Arg-AFC were hydrolyzed when incubated with spent media from the oncospheres compared to control culture medium removed prior to incubation. Hydrolysis of Z-Phe-Arg-AFC was inhibited 78% by preincubation of the medium with the serine proteinase inhibitor Phenylmethylsulfonyl fluoride. Endopeptidase activity was partially enhanced in the presence of exogenous thiols and partially inhibited with the cysteine proteinase inhibitor E-64, suggesting the presence of both serine and cysteine endopeptidases. No significant inhibition was noted with pepstatin or phenanthroline. The peptidase activities detected with Z-Phe-Arg-AFC and Arg- AFC were separated by gel-filtration fast protein liquid chromatography and eluted at volumes corresponding to molecular weights of 18 and 30 kDa, respectively. These data demonstrate that T. saginata oncospheres produce excretory/secretory peptidases, including serine and cysteine endopeptidases and an aminopeptidase. These enzymes may play a role in invasion of the intestinal mucosa of the intermediate host.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalJournal of Parasitology
Volume82
Issue number1
DOIs
StatePublished - Feb 1996
Externally publishedYes

Fingerprint

Taenia saginata
oncospheres
coumarin
peptidases
Peptide Hydrolases
substrate
inhibitor
Serine Endopeptidases
intermediate host
serine proteinases
thiol
fluoride
peptide
Endopeptidases
aminopeptidases
liquid chromatography
hydrolysis
cysteine proteinases
gel
incubation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Taenia saginata oncosphere excretory/secretory peptidases. / White, A. Clinton; Baig, Salman; Robinson, Prema.

In: Journal of Parasitology, Vol. 82, No. 1, 02.1996, p. 7-10.

Research output: Contribution to journalArticle

White, A. Clinton ; Baig, Salman ; Robinson, Prema. / Taenia saginata oncosphere excretory/secretory peptidases. In: Journal of Parasitology. 1996 ; Vol. 82, No. 1. pp. 7-10.
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