Abstract
Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 141-157 |
| Number of pages | 17 |
| Volume | 1523 |
| DOIs | |
| State | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1523 |
| ISSN (Print) | 10643745 |
Keywords
- Amyloid seeding
- Tau oligomer isolation
- Tau oligomers
- Tauopathies
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Gerson, J. E., Sengupta, U., & Kayed, R. (2017). Tau oligomers as pathogenic seeds: Preparation and propagation in vitro and in vivo. In Methods in Molecular Biology (Vol. 1523, pp. 141-157). (Methods in Molecular Biology; Vol. 1523). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6598-4_9