Abstract
Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 141-157 |
Number of pages | 17 |
Volume | 1523 |
DOIs | |
State | Published - 2017 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1523 |
ISSN (Print) | 10643745 |
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Keywords
- Amyloid seeding
- Tau oligomer isolation
- Tau oligomers
- Tauopathies
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Tau oligomers as pathogenic seeds : Preparation and propagation in vitro and in vivo. / Gerson, Julia E.; Sengupta, Urmi; Kayed, Rakez.
Methods in Molecular Biology. Vol. 1523 Humana Press Inc., 2017. p. 141-157 (Methods in Molecular Biology; Vol. 1523).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Tau oligomers as pathogenic seeds
T2 - Preparation and propagation in vitro and in vivo
AU - Gerson, Julia E.
AU - Sengupta, Urmi
AU - Kayed, Rakez
PY - 2017
Y1 - 2017
N2 - Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
AB - Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
KW - Amyloid seeding
KW - Tau oligomer isolation
KW - Tau oligomers
KW - Tauopathies
UR - http://www.scopus.com/inward/record.url?scp=85006508536&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006508536&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6598-4_9
DO - 10.1007/978-1-4939-6598-4_9
M3 - Chapter
C2 - 27975249
AN - SCOPUS:85006508536
VL - 1523
T3 - Methods in Molecular Biology
SP - 141
EP - 157
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -