Tau oligomers as pathogenic seeds: Preparation and propagation in vitro and in vivo

Julia E. Gerson; Urmi Sengupta; Rakez Kayed

doi:10.1007/978-1-4939-6598-4_9

Tau oligomers as pathogenic seeds: Preparation and propagation in vitro and in vivo

Julia E. Gerson, Urmi Sengupta, Rakez Kayed

Neurology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Scopus citations

Abstract

Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	141-157
Number of pages	17
Volume	1523
DOIs	https://doi.org/10.1007/978-1-4939-6598-4_9
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1523
ISSN (Print)	10643745

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Keywords

Amyloid seeding
Tau oligomer isolation
Tau oligomers
Tauopathies

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Gerson, J. E., Sengupta, U., & Kayed, R. (2017). Tau oligomers as pathogenic seeds: Preparation and propagation in vitro and in vivo. In Methods in Molecular Biology (Vol. 1523, pp. 141-157). (Methods in Molecular Biology; Vol. 1523). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6598-4_9

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Access to Document

10.1007/978-1-4939-6598-4_9