Telomere staining of human chromosomes and the mechanism of radiation-induced dicentric formation

Michael Cornforth, J. Meyne, L. G. Littlefield, S. M. Bailey, R. K. Moyzis

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The majority of models of radiation action developed over the past half century hold that the curvilinear dose responses exhibited by eukaryotic cells to sparsely ionizing radiations result from the interaction of pairs of lesions produced in sensitive targets of the cell. Within this conceptual framework, chromosomal exchange aberrations (e.g., interchanges) are believed to occur through the interaction of damaged sites on both chromosomes participating in the exchange. In contrast, the model proposed by Chadwick and Leenhouts (as well as some other models) suggests that such exchanges arise from initial radiation damage to only one chromosome, which then becomes associated with an undamaged chromosome. A particular aspect of this theory is that asymmetrical exchanges, such as dicentrics, may be formed from the rejoining of a broken end of one chromosome to the telomere of another. By using a DNA probe that specifically hybridizes to the telomeric region of human chromosomes, we were able to test this assertion directly. After scanning more than 200 dicentrics produced in normal human fibroblasts by 6 Gy of 60Co γ rays, virtually none were found that contained telomeres located between the centromeres of this aberration type. Therefore, since the proposed telomere-break rejoining process, per se, is not necessarily a central element of the Chadwick-Leenhouts model, we suggest the theory be modified to exclude this mechanism.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalRadiation Research
Volume120
Issue number2
StatePublished - 1989
Externally publishedYes

Fingerprint

telomeres
chromosomes
Telomere
Human Chromosomes
staining
Chromosomes
Radiation
Staining and Labeling
radiation
aberration
Centromere
DNA Probes
Eukaryotic Cells
Ionizing Radiation
Chromosome Aberrations
DNA probes
fibroblasts
centromeres
ionizing radiation
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Cornforth, M., Meyne, J., Littlefield, L. G., Bailey, S. M., & Moyzis, R. K. (1989). Telomere staining of human chromosomes and the mechanism of radiation-induced dicentric formation. Radiation Research, 120(2), 205-212.

Telomere staining of human chromosomes and the mechanism of radiation-induced dicentric formation. / Cornforth, Michael; Meyne, J.; Littlefield, L. G.; Bailey, S. M.; Moyzis, R. K.

In: Radiation Research, Vol. 120, No. 2, 1989, p. 205-212.

Research output: Contribution to journalArticle

Cornforth, M, Meyne, J, Littlefield, LG, Bailey, SM & Moyzis, RK 1989, 'Telomere staining of human chromosomes and the mechanism of radiation-induced dicentric formation', Radiation Research, vol. 120, no. 2, pp. 205-212.
Cornforth, Michael ; Meyne, J. ; Littlefield, L. G. ; Bailey, S. M. ; Moyzis, R. K. / Telomere staining of human chromosomes and the mechanism of radiation-induced dicentric formation. In: Radiation Research. 1989 ; Vol. 120, No. 2. pp. 205-212.
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