Temperature alters Plasmodium blocking by Wolbachia

Courtney C. Murdock, Simon Blanford, Grant L. Hughes, Jason L. Rasgon, Matthew B. Thomas

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Very recently, the Asian malaria vector (Anopheles stephensi) was stably transinfected with the wAlbB strain of Wolbachia, inducing refractoriness to the human malaria parasite Plasmodium falciparum. However, conditions in the field can differ substantially from those in the laboratory. We use the rodent malaria P. yoelii, and somatically transinfected An. stephensi as a model system to investigate whether the transmission blocking potential of wAlbB is likely to be robust across different thermal environments. wAlbB reduced malaria parasite prevalence and oocyst intensity at 28 C. At 24 C there was no effect on prevalence but a marked increase in oocyst intensity. At 20 C, wAlbB had no effect on prevalence or intensity. Additionally, we identified a novel effect of wAlbB that resulted in reduced sporozoite development across temperatures, counterbalancing the oocyst enhancement at 24 C. Our results demonstrate complex effects of temperature on the Wolbachia-malaria interaction, and suggest the impacts of transinfection might vary across diverse environments.

Original languageEnglish (US)
Article number3932
JournalScientific Reports
Volume4
DOIs
StatePublished - Feb 3 2014
Externally publishedYes

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Wolbachia
Plasmodium
Malaria
Oocysts
Temperature
Parasites
Sporozoites
Anopheles
Falciparum Malaria
Rodentia
Hot Temperature

ASJC Scopus subject areas

  • General

Cite this

Murdock, C. C., Blanford, S., Hughes, G. L., Rasgon, J. L., & Thomas, M. B. (2014). Temperature alters Plasmodium blocking by Wolbachia. Scientific Reports, 4, [3932]. https://doi.org/10.1038/srep03932

Temperature alters Plasmodium blocking by Wolbachia. / Murdock, Courtney C.; Blanford, Simon; Hughes, Grant L.; Rasgon, Jason L.; Thomas, Matthew B.

In: Scientific Reports, Vol. 4, 3932, 03.02.2014.

Research output: Contribution to journalArticle

Murdock, CC, Blanford, S, Hughes, GL, Rasgon, JL & Thomas, MB 2014, 'Temperature alters Plasmodium blocking by Wolbachia', Scientific Reports, vol. 4, 3932. https://doi.org/10.1038/srep03932
Murdock CC, Blanford S, Hughes GL, Rasgon JL, Thomas MB. Temperature alters Plasmodium blocking by Wolbachia. Scientific Reports. 2014 Feb 3;4. 3932. https://doi.org/10.1038/srep03932
Murdock, Courtney C. ; Blanford, Simon ; Hughes, Grant L. ; Rasgon, Jason L. ; Thomas, Matthew B. / Temperature alters Plasmodium blocking by Wolbachia. In: Scientific Reports. 2014 ; Vol. 4.
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