Temperature and cell-type dependency of sulfide effects on mitochondrial respiration

Michael Groeger, Jose Matallo, Oscar McCook, Florian Wagner, Ulrich Wachter, Olga Bastian, Saskia Gierer, Vera Reich, Bettina Stahl, Markus Huber-Lang, Csaba Szabo, Michael Georgieff, Peter Radermacher, Enrico Calzia, Katja Wagner

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Previous studies suggest that sulfide-induced inhibition of cytochrome c oxidase (cCox) and, consequently, the metabolic and toxic effects of sulfide are less pronounced at low body temperature. Because the temperature-dependent effects of sulfide on the inflammatory response are still a matter of debate, we investigated the impact of varying temperature on the cCox excess capacity and the mitochondrial sulfide oxidation by the sulfide-ubiquinone oxidoreductase in macrophage-derived cell lines (AMJ2-C11 and RAW 264.7). Using an oxygraph chamber, the inhibition of mitochondrial respiration was measured by stepwise titrations with sulfide and the nonmetabolizable cCox inhibitor sodium azide at 25°C and 37°C. Using the latter of the two inhibitors, the excess capacity of the cCox was obtained. Furthermore, we quantified the capacity of these cells to withstand sulfide inhibition by measuring the amount required to inhibit respiration by 50% and 90% and the viability of the cells after 24-h exposure to 100 ppm of hydrogen sulfide. At low titration rates, the AMJ2-C11 cells, but not the RAW 264.7 cells, increased their capacity to withstand exogenously added sulfide. This effect was even greater at 25°C than at 37°C. Furthermore, only the AMJ2-C11 cells remained viable after sulfide exposure for 24 h. In contrast, only in the RAW 264.7 cells that an increase in cCox excess capacity was found at low temperatures. In macrophage-derived cell lines, both the excess capacity of cCox and the efficiency of sulfide elimination may increase at low temperatures. These properties may modify the effects of sulfide in immune cells and, potentially, the inflammatory response during sulfide exposure at different body temperatures.

Original languageEnglish (US)
Pages (from-to)367-374
Number of pages8
JournalShock
Volume38
Issue number4
DOIs
StatePublished - Oct 2012

Fingerprint

Sulfides
Respiration
Temperature
Electron Transport Complex IV
Body Temperature
Macrophages
Cell Line
Sodium Azide
Hydrogen Sulfide
Ubiquinone
Poisons
Cell Survival
Oxidoreductases

Keywords

  • cytochrome c oxidase
  • Hydrogen sulfide
  • suspended animation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Groeger, M., Matallo, J., McCook, O., Wagner, F., Wachter, U., Bastian, O., ... Wagner, K. (2012). Temperature and cell-type dependency of sulfide effects on mitochondrial respiration. Shock, 38(4), 367-374. https://doi.org/10.1097/SHK.0b013e3182651fe6

Temperature and cell-type dependency of sulfide effects on mitochondrial respiration. / Groeger, Michael; Matallo, Jose; McCook, Oscar; Wagner, Florian; Wachter, Ulrich; Bastian, Olga; Gierer, Saskia; Reich, Vera; Stahl, Bettina; Huber-Lang, Markus; Szabo, Csaba; Georgieff, Michael; Radermacher, Peter; Calzia, Enrico; Wagner, Katja.

In: Shock, Vol. 38, No. 4, 10.2012, p. 367-374.

Research output: Contribution to journalArticle

Groeger, M, Matallo, J, McCook, O, Wagner, F, Wachter, U, Bastian, O, Gierer, S, Reich, V, Stahl, B, Huber-Lang, M, Szabo, C, Georgieff, M, Radermacher, P, Calzia, E & Wagner, K 2012, 'Temperature and cell-type dependency of sulfide effects on mitochondrial respiration', Shock, vol. 38, no. 4, pp. 367-374. https://doi.org/10.1097/SHK.0b013e3182651fe6
Groeger M, Matallo J, McCook O, Wagner F, Wachter U, Bastian O et al. Temperature and cell-type dependency of sulfide effects on mitochondrial respiration. Shock. 2012 Oct;38(4):367-374. https://doi.org/10.1097/SHK.0b013e3182651fe6
Groeger, Michael ; Matallo, Jose ; McCook, Oscar ; Wagner, Florian ; Wachter, Ulrich ; Bastian, Olga ; Gierer, Saskia ; Reich, Vera ; Stahl, Bettina ; Huber-Lang, Markus ; Szabo, Csaba ; Georgieff, Michael ; Radermacher, Peter ; Calzia, Enrico ; Wagner, Katja. / Temperature and cell-type dependency of sulfide effects on mitochondrial respiration. In: Shock. 2012 ; Vol. 38, No. 4. pp. 367-374.
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