Temperature-induced changes in photosynthetic activities and thylakoid membrane properties of Euglena gracilis

Shude Zhong, William G. Nolan, Pei-Yong Shi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Euglena gracilis Klebs strain Z Pringsheim was grown at 26°C, and the effects of temperature on photosynthesis and thylakoid parameters were determined. Arrhenius plots of oxygen evolution accompanying CO2 or p-benzoquinone reduction by intact cells showed a discontinuity at approx. 6-7°C. Plots of oxygen evolution accompanying ferricyanide or benzoquinone reduction by isolated thylakoids showed a discontinuity at approximately 5.5-7°C. A high sensitivity differential scanning calorimetric study of isolated thylakoids showed that a very sharp and reversible transition occurred at 6.2°C. We conclude that a temperature-induced change in the structure of thylakoid lipids resulted in the discontinuity observed in the Arrhenius plots. Calorimetric experiments also demonstrated that several transitions occurred at temperatures higher than 20°C with one at approx. 63°C being the most prominent. As in the case of thylakoids of most higher plants, all of the higher temperature transitions were irreversible.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalPlant Science
Volume92
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Euglena gracilis
Thylakoids
thylakoids
Arrhenius plots
Membranes
Temperature
benzoquinones
Oxygen
temperature
Photosynthesis
ferricyanides
Transition Temperature
Scanning
Lipids
photosynthesis
Experiments
lipids
benzoquinone
cells

Keywords

  • Differential scanning calorimetry
  • Electron transport
  • Euglena gracilis
  • Photosynthesis
  • Temperature
  • Thylakoid structure and function

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Temperature-induced changes in photosynthetic activities and thylakoid membrane properties of Euglena gracilis. / Zhong, Shude; Nolan, William G.; Shi, Pei-Yong.

In: Plant Science, Vol. 92, No. 2, 1993, p. 121-127.

Research output: Contribution to journalArticle

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N2 - Euglena gracilis Klebs strain Z Pringsheim was grown at 26°C, and the effects of temperature on photosynthesis and thylakoid parameters were determined. Arrhenius plots of oxygen evolution accompanying CO2 or p-benzoquinone reduction by intact cells showed a discontinuity at approx. 6-7°C. Plots of oxygen evolution accompanying ferricyanide or benzoquinone reduction by isolated thylakoids showed a discontinuity at approximately 5.5-7°C. A high sensitivity differential scanning calorimetric study of isolated thylakoids showed that a very sharp and reversible transition occurred at 6.2°C. We conclude that a temperature-induced change in the structure of thylakoid lipids resulted in the discontinuity observed in the Arrhenius plots. Calorimetric experiments also demonstrated that several transitions occurred at temperatures higher than 20°C with one at approx. 63°C being the most prominent. As in the case of thylakoids of most higher plants, all of the higher temperature transitions were irreversible.

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