Temporal analysis of human leucocyte surface antigen expression and neutrophil respiratory burst activity after thermal injury

Ramon Zapata Sirvent, J. F. Hansbrough

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Sepsis, the major cause of morlidity and mortality after burn injury, is related to multiple immune derangements. Using monoclonal antibodies and two-colour flow cytometry to identify surface antigens, peripheral blood mononuclear cell (PBMC) populations were analysed and correlated with lymphocyte proliferation assays for 21 days postinjury. In addition, in vitro expression of activation antigens by mitogen-stimulated PBMCs was analysed during the time period. Twenty-nine burn patients were studied, with burn injuries ranging from 19 to 97 per cent TBSA; PBMCs from human volunteers were used for control cells. Patients received aggressive enteral nutritional support starting on day 1 postburn and underwent early excision and grafting of wounds; no patients developed sepsis during the study period. The most consistent changes in PBMCs after thermal injury were decreased percentages of total T cells (CD3+), T helper/inducer cells (CD4+), and T suppressor/cytotoxic cells (CD8+); the percentages of natural killer (CD16+ ) cells were not altered. Expression of surface 'activation' antigens on CD4+ and CD8+ cells (HLA-DR, interleukin-2 receptor and transferrin receptor) after mitogen stimulation was significantly depressed as early as 1 day postburn. An early monocytosis was seen on day 1 postburn, but decreases were found on days 4 and 7. Monocyte expression of HLA-DR antigen was suppressed throughout the study. Lymphocyte proliferation after mitogen stimulation and the responses of lymphocytes in mixed lymphocyte culture were suppressed postburn. Neutrophil respiratory burst responses were supranormal on days 1 and 7 postburn, but the differences were not statistically significant. Although burn morbidity and mortality are primarily due to bacterial organisms, defects in T-cell and monocyte immune functions are more predominant than defects in neutrophil bactericidal function in these patients. The block in T-cell blastogenesis is early in the cell cycle, prior to DNA synthesis.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalBurns
Volume19
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Respiratory Burst
Surface Antigens
HLA Antigens
Neutrophils
Hot Temperature
Mitogens
Wounds and Injuries
HLA-DR Antigens
Lymphocytes
Lymphocyte Activation
T-Lymphocytes
Monocytes
Sepsis
Transferrin Receptors
Nutritional Support
Mortality
Interleukin-2 Receptors
Helper-Inducer T-Lymphocytes
Natural Killer Cells
Small Intestine

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Temporal analysis of human leucocyte surface antigen expression and neutrophil respiratory burst activity after thermal injury. / Zapata Sirvent, Ramon; Hansbrough, J. F.

In: Burns, Vol. 19, No. 1, 01.01.1993, p. 5-11.

Research output: Contribution to journalArticle

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