Abstract
BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.
METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.
RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.
CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1638-1647 |
| Number of pages | 10 |
| Journal | Head and Neck |
| Volume | 36 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 2014 |
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Keywords
- head and neck squamous cell carcinoma
- lymph node metastasis
- oral cancer
- orthotopic xenograft mouse model
- physiologic characterization
ASJC Scopus subject areas
- Medicine(all)
Cite this
Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer. / Szaniszlo, Peter; Fennewald, Susan M.; Qiu, Suimin; Kantara, Carla; Shilagard, Tuya; Vargas, Gracie; Resto, Vicente.
In: Head and Neck, Vol. 36, No. 11, 01.11.2014, p. 1638-1647.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer
AU - Szaniszlo, Peter
AU - Fennewald, Susan M.
AU - Qiu, Suimin
AU - Kantara, Carla
AU - Shilagard, Tuya
AU - Vargas, Gracie
AU - Resto, Vicente
PY - 2014/11/1
Y1 - 2014/11/1
N2 - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
AB - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
KW - head and neck squamous cell carcinoma
KW - lymph node metastasis
KW - oral cancer
KW - orthotopic xenograft mouse model
KW - physiologic characterization
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U2 - 10.1002/hed.23500
DO - 10.1002/hed.23500
M3 - Article
VL - 36
SP - 1638
EP - 1647
JO - Head and Neck Surgery
JF - Head and Neck Surgery
SN - 1043-3074
IS - 11
ER -