Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer

Peter Szaniszlo; Susan M. Fennewald; Suimin Qiu; Carla Kantara; Tuya Shilagard; Gracie Vargas; Vicente Resto

doi:10.1002/hed.23500

Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer

Peter Szaniszlo, Susan M. Fennewald, Suimin Qiu, Carla Kantara, Tuya Shilagard, Gracie Vargas, Vicente Resto

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.

METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.

RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.

CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

Original language	English (US)
Pages (from-to)	1638-1647
Number of pages	10
Journal	Head and Neck
Volume	36
Issue number	11
DOIs	https://doi.org/10.1002/hed.23500
State	Published - Nov 1 2014

Keywords

head and neck squamous cell carcinoma
lymph node metastasis
oral cancer
orthotopic xenograft mouse model
physiologic characterization

ASJC Scopus subject areas

Medicine(all)

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Szaniszlo, P., Fennewald, S. M., Qiu, S., Kantara, C., Shilagard, T., Vargas, G., & Resto, V. (2014). Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer. Head and Neck, 36(11), 1638-1647. https://doi.org/10.1002/hed.23500

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abstract = "BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.",

keywords = "head and neck squamous cell carcinoma, lymph node metastasis, oral cancer, orthotopic xenograft mouse model, physiologic characterization",

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AU - Szaniszlo, Peter

AU - Fennewald, Susan M.

AU - Qiu, Suimin

AU - Kantara, Carla

AU - Shilagard, Tuya

AU - Vargas, Gracie

AU - Resto, Vicente

PY - 2014/11/1

Y1 - 2014/11/1

N2 - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

AB - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

KW - head and neck squamous cell carcinoma

KW - lymph node metastasis

KW - oral cancer

KW - orthotopic xenograft mouse model

KW - physiologic characterization

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