Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer

Peter Szaniszlo, Susan M. Fennewald, Suimin Qiu, Carla Kantara, Tuya Shilagard, Gracie Vargas, Vicente Resto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.

METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.

RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.

CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

Original languageEnglish (US)
Pages (from-to)1638-1647
Number of pages10
JournalHead and Neck
Volume36
Issue number11
DOIs
StatePublished - Nov 1 2014

Fingerprint

Lymphatic Metastasis
Mouth Neoplasms
Heterografts
Nude Mice
Histology
Lymph Nodes
Neoplasm Metastasis
Cell Line
Neoplasms
Treatment Failure
Photons
Tongue
Disease Progression
Microscopy
Animal Models
Immunohistochemistry
Injections
Survival
Mortality
Carcinoma, squamous cell of head and neck

Keywords

  • head and neck squamous cell carcinoma
  • lymph node metastasis
  • oral cancer
  • orthotopic xenograft mouse model
  • physiologic characterization

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer. / Szaniszlo, Peter; Fennewald, Susan M.; Qiu, Suimin; Kantara, Carla; Shilagard, Tuya; Vargas, Gracie; Resto, Vicente.

In: Head and Neck, Vol. 36, No. 11, 01.11.2014, p. 1638-1647.

Research output: Contribution to journalArticle

Szaniszlo, Peter ; Fennewald, Susan M. ; Qiu, Suimin ; Kantara, Carla ; Shilagard, Tuya ; Vargas, Gracie ; Resto, Vicente. / Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer. In: Head and Neck. 2014 ; Vol. 36, No. 11. pp. 1638-1647.
@article{036e73cc4a0c4181b96dd8a439f46f2f,
title = "Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer",
abstract = "BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.",
keywords = "head and neck squamous cell carcinoma, lymph node metastasis, oral cancer, orthotopic xenograft mouse model, physiologic characterization",
author = "Peter Szaniszlo and Fennewald, {Susan M.} and Suimin Qiu and Carla Kantara and Tuya Shilagard and Gracie Vargas and Vicente Resto",
year = "2014",
month = "11",
day = "1",
doi = "10.1002/hed.23500",
language = "English (US)",
volume = "36",
pages = "1638--1647",
journal = "Head and Neck Surgery",
issn = "1043-3074",
publisher = "Wiley-Liss Inc.",
number = "11",

}

TY - JOUR

T1 - Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer

AU - Szaniszlo, Peter

AU - Fennewald, Susan M.

AU - Qiu, Suimin

AU - Kantara, Carla

AU - Shilagard, Tuya

AU - Vargas, Gracie

AU - Resto, Vicente

PY - 2014/11/1

Y1 - 2014/11/1

N2 - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

AB - BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.

KW - head and neck squamous cell carcinoma

KW - lymph node metastasis

KW - oral cancer

KW - orthotopic xenograft mouse model

KW - physiologic characterization

UR - http://www.scopus.com/inward/record.url?scp=84933525782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933525782&partnerID=8YFLogxK

U2 - 10.1002/hed.23500

DO - 10.1002/hed.23500

M3 - Article

VL - 36

SP - 1638

EP - 1647

JO - Head and Neck Surgery

JF - Head and Neck Surgery

SN - 1043-3074

IS - 11

ER -