Temporal global changes in gene expression during temperature transition in Yersinia pestis

Vladimir Motin, Anca M. Georgescu, Joseph P. Fitch, Pauline P. Gu, David O. Nelson, Shalini L. Mabery, Janine B. Garnham, Bahrad A. Sokhansanj, Linda L. Ott, Matthew A. Coleman, Jeffrey M. Elliott, Laura M. Kegelmeyer, Andrew J. Wyrobek, Thomas R. Slezak, Robert R. Brubaker, Emilio Garcia

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37°C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca2+. In contrast, Ca2+ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.

Original languageEnglish (US)
Pages (from-to)6298-6305
Number of pages8
JournalJournal of Bacteriology
Volume186
Issue number18
DOIs
StatePublished - Sep 2004

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Yersinia pestis
Transition Temperature
Gene Expression
Open Reading Frames
Down-Regulation
Genes
Persistent Vegetative State
Oligonucleotide Array Sequence Analysis
Genome
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Motin, V., Georgescu, A. M., Fitch, J. P., Gu, P. P., Nelson, D. O., Mabery, S. L., ... Garcia, E. (2004). Temporal global changes in gene expression during temperature transition in Yersinia pestis. Journal of Bacteriology, 186(18), 6298-6305. https://doi.org/10.1128/JB.186.18.6298-6305.2004

Temporal global changes in gene expression during temperature transition in Yersinia pestis. / Motin, Vladimir; Georgescu, Anca M.; Fitch, Joseph P.; Gu, Pauline P.; Nelson, David O.; Mabery, Shalini L.; Garnham, Janine B.; Sokhansanj, Bahrad A.; Ott, Linda L.; Coleman, Matthew A.; Elliott, Jeffrey M.; Kegelmeyer, Laura M.; Wyrobek, Andrew J.; Slezak, Thomas R.; Brubaker, Robert R.; Garcia, Emilio.

In: Journal of Bacteriology, Vol. 186, No. 18, 09.2004, p. 6298-6305.

Research output: Contribution to journalArticle

Motin, V, Georgescu, AM, Fitch, JP, Gu, PP, Nelson, DO, Mabery, SL, Garnham, JB, Sokhansanj, BA, Ott, LL, Coleman, MA, Elliott, JM, Kegelmeyer, LM, Wyrobek, AJ, Slezak, TR, Brubaker, RR & Garcia, E 2004, 'Temporal global changes in gene expression during temperature transition in Yersinia pestis', Journal of Bacteriology, vol. 186, no. 18, pp. 6298-6305. https://doi.org/10.1128/JB.186.18.6298-6305.2004
Motin, Vladimir ; Georgescu, Anca M. ; Fitch, Joseph P. ; Gu, Pauline P. ; Nelson, David O. ; Mabery, Shalini L. ; Garnham, Janine B. ; Sokhansanj, Bahrad A. ; Ott, Linda L. ; Coleman, Matthew A. ; Elliott, Jeffrey M. ; Kegelmeyer, Laura M. ; Wyrobek, Andrew J. ; Slezak, Thomas R. ; Brubaker, Robert R. ; Garcia, Emilio. / Temporal global changes in gene expression during temperature transition in Yersinia pestis. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 18. pp. 6298-6305.
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