Testing Antiviral Compounds in a Dengue Mouse Model

Wouter Schul, Andy Yip, Pei-Yong Shi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Dengue fever is an emerging mosquito-borne flaviviral disease that threatens 2.5 billion people worldwide. No clinically approved vaccine and antiviral therapy are currently available to prevent or treat dengue virus (DENV) infection. Vertebrate animals other than primates are not normally infectable with DENV; however, a small animal dengue infection model would greatly facilitate the development of a vaccine or an antiviral therapy. To this end, a rodent model for DENV infection has been established in IFN-α/β and IFN-γ receptor-deficient (AG129) mice. This chapter describes the protocol for the DENV infection model in AG129 mice and testing of antiviral compounds by oral gavage or parenteral injection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages269-281
Number of pages13
Volume1030
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1030
ISSN (Print)10643745

Fingerprint

Dengue Virus
Dengue
Antiviral Agents
Virus Diseases
Active Immunotherapy
Culicidae
Primates
Vertebrates
Rodentia
Vaccines
Injections
Infection
Therapeutics

Keywords

  • Animal model
  • Antiviral testing
  • Dengue virus
  • Flavivirus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Schul, W., Yip, A., & Shi, P-Y. (2013). Testing Antiviral Compounds in a Dengue Mouse Model. In Methods in Molecular Biology (Vol. 1030, pp. 269-281). (Methods in Molecular Biology; Vol. 1030). https://doi.org/10.1007/978-1-62703-484-5_21

Testing Antiviral Compounds in a Dengue Mouse Model. / Schul, Wouter; Yip, Andy; Shi, Pei-Yong.

Methods in Molecular Biology. Vol. 1030 2013. p. 269-281 (Methods in Molecular Biology; Vol. 1030).

Research output: Chapter in Book/Report/Conference proceedingChapter

Schul, W, Yip, A & Shi, P-Y 2013, Testing Antiviral Compounds in a Dengue Mouse Model. in Methods in Molecular Biology. vol. 1030, Methods in Molecular Biology, vol. 1030, pp. 269-281. https://doi.org/10.1007/978-1-62703-484-5_21
Schul W, Yip A, Shi P-Y. Testing Antiviral Compounds in a Dengue Mouse Model. In Methods in Molecular Biology. Vol. 1030. 2013. p. 269-281. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-484-5_21
Schul, Wouter ; Yip, Andy ; Shi, Pei-Yong. / Testing Antiviral Compounds in a Dengue Mouse Model. Methods in Molecular Biology. Vol. 1030 2013. pp. 269-281 (Methods in Molecular Biology).
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