Testing Antiviral Compounds in a Dengue Mouse Model

Wouter Schul, Andy Yip, Pei Yong Shi

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Dengue fever is an emerging mosquito-borne flaviviral disease that threatens 2.5 billion people worldwide. No clinically approved vaccine and antiviral therapy are currently available to prevent or treat dengue virus (DENV) infection. Vertebrate animals other than primates are not normally infectable with DENV; however, a small animal dengue infection model would greatly facilitate the development of a vaccine or an antiviral therapy. To this end, a rodent model for DENV infection has been established in IFN-α/β and IFN-γ receptor-deficient (AG129) mice. This chapter describes the protocol for the DENV infection model in AG129 mice and testing of antiviral compounds by oral gavage or parenteral injection.

Original languageEnglish (US)
Title of host publicationAntiviral Methods and Protocols
Pages269-281
Number of pages13
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1030
ISSN (Print)1064-3745

Keywords

  • Animal model
  • Antiviral testing
  • Dengue virus
  • Flavivirus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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