Testing the notion of the one-hit exchange

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Classical theory asserts that radiation-induced chromosomal exchanges result from the interaction of lesions on both chromosomes involved, a notion supported by substantial indirect evidence, but more recently questioned on biophysical and molecular grounds. When mitotic HeLa cells were irradiated with 60Co γ rays, and fused together with Sendai virus, numerous chromosome exchanges were observed between the genomes of different cells at the next mitosis. However, when irradiated and unirradiated cells were fused together, the frequency of intergenomic exchange was 40-fold lower, suggesting that the vast majority of radiation-induced exchanges do, in fact, require damage to both chromosomes.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalRadiation Research
Volume121
Issue number1
StatePublished - 1990
Externally publishedYes

Fingerprint

chromosomes
Chromosomes
cells
Radiation
Sendai virus
mitosis
genome
testing
viruses
radiation
HeLa Cells
Mitosis
lesions
rays
Genome
damage
interactions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Testing the notion of the one-hit exchange. / Cornforth, Michael.

In: Radiation Research, Vol. 121, No. 1, 1990, p. 21-27.

Research output: Contribution to journalArticle

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