Testosterone injection stimulates net protein synthesis but not tissue amino acid transport

Arny A. Ferrando, Kevin D. Tipton, David Doyle, Stuart M. Phillips, Joaquin Cortiella, Robert R. Wolfe

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

Testosterone administration (T) increases lean body mass and muscle protein synthesis. We investigated the effects of short-term T on leg muscle protein kinetics and transport of selected amino acids by use of a model based on arteriovenous sampling and muscle biopsy. Fractional synthesis (FSR) and breakdown (FBR) rates of skeletal muscle protein were also directly calculated. Seven healthy men were studied before and 5 days after intramuscular injection of 200 mg of testosterone enanthate. Protein synthesis increased twofold after injection (P < 0.05), whereas protein breakdown was unchanged. FSR and FBR calculations were in accordance, because FSR increased twofold (P < 0.05) without a concomitant change in FBR. Net balance between synthesis and breakdown became more positive with both methodologies (P < 0.05) and was not different from zero. T injection increased arteriovenous essential and nonessential nitrogen balance across the leg (P < 0.05) in the fasted state, without increasing amino acid transport. Thus T administration leads to an increased net protein synthesis and reutilization of intracellular amino acids in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)E864-E871
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume275
Issue number5 38-5
DOIs
StatePublished - Jan 1 1998

Keywords

  • Amino nitrogen uptake
  • Fractional breakdown rate

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Testosterone injection stimulates net protein synthesis but not tissue amino acid transport'. Together they form a unique fingerprint.

  • Cite this