TY - JOUR
T1 - Testosterone upregulation of tissue type plasminogen activator expression in Sertoli cells
T2 - tPA expression in Sertoli cells
AU - Guo, Jian
AU - Shi, Yu Qiang
AU - Yang, Wei
AU - Li, Yin Chuan
AU - Hu, Zhao Yuan
AU - Liu, Yi Xun
N1 - Funding Information:
Acknowledgments This study was supported by the ‘‘973’’ project (2006CB504001), the Major Research Plan (2006CB944001); the CAS Innovation Project (KSCA2-YW-R-55), the National Nature Science Foundation of China (No: 30618005;); and Beijing Nature Science Foundation (5073032).
PY - 2007/8
Y1 - 2007/8
N2 - Our previous studies have demonstrated that tissue type plasminogen activator (tPA) might be involved in matrix degradation of blood-testis barrier in rat. In this study, we have further investigated the effect of testosterone (T) on tPA production in rat Sertoli cells. Our results showed that Sertoli cells isolated from rat testes at various ages in vitro secreted tPA in an age-dependent manner. The tPA activity was detected on day 20 after birth, and reached maximum on day 60. The Sertoli cells isolated from the testes on day 20 were then cultured in the presence or absence of testosterone, FSH, and forskolin, the tPA activities were upregulated by T, FSH and forskolin. Addition of H89 or U0126, both inhibited the testosterone-, FSH-, and forskolin-induced tPA expression. It is suggested that FSH- and testosterone-stimulated tPA expression in Sertoli cells may be via PKA and ERK signal transduction. Furthermore, we have observed that testosterone stimulated tPA secretion at all the stages of spermatogenesis (II-VI, VII-VIII, IX-XII and XIII-I), the highest stimulation of tPA activity was observed at stages VII-VIII. This study further suggests that testosterone-induced tPA activity in the Sertoli cells might be related to the function of blood-testis barrier opening and/or closing.
AB - Our previous studies have demonstrated that tissue type plasminogen activator (tPA) might be involved in matrix degradation of blood-testis barrier in rat. In this study, we have further investigated the effect of testosterone (T) on tPA production in rat Sertoli cells. Our results showed that Sertoli cells isolated from rat testes at various ages in vitro secreted tPA in an age-dependent manner. The tPA activity was detected on day 20 after birth, and reached maximum on day 60. The Sertoli cells isolated from the testes on day 20 were then cultured in the presence or absence of testosterone, FSH, and forskolin, the tPA activities were upregulated by T, FSH and forskolin. Addition of H89 or U0126, both inhibited the testosterone-, FSH-, and forskolin-induced tPA expression. It is suggested that FSH- and testosterone-stimulated tPA expression in Sertoli cells may be via PKA and ERK signal transduction. Furthermore, we have observed that testosterone stimulated tPA secretion at all the stages of spermatogenesis (II-VI, VII-VIII, IX-XII and XIII-I), the highest stimulation of tPA activity was observed at stages VII-VIII. This study further suggests that testosterone-induced tPA activity in the Sertoli cells might be related to the function of blood-testis barrier opening and/or closing.
KW - Blood-testis barrier
KW - ERK pathway
KW - PKA
KW - Testosterone
KW - Tissue-type plasminogen activator
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U2 - 10.1007/s12020-007-9014-1
DO - 10.1007/s12020-007-9014-1
M3 - Article
C2 - 17992606
AN - SCOPUS:36949029383
SN - 1355-008X
VL - 32
SP - 83
EP - 89
JO - Endocrine
JF - Endocrine
IS - 1
ER -