Tetraacylated lipopolysaccharide of yersinia pestis can inhibit multiple toll-like receptor-mediated signaling pathways in human dendritic cells

Maxim V. Telepnev, Gary R. Klimpel, Judith Haithcoat, Yuriy A. Knirel, Andrey P. Anisimov, Vladimir Motin

Research output: Contribution to journalArticle

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Abstract

Background: Yersinia pestis, the causative agent of plague, showed a temperature-dependent change in lipid A composition, with a reduced degree of acylation when bacteria were grown at 37°C (tetraacylated) versus ambient temperature (hexaacylated). Methods: Human monocytes and monocyte-derived dendritic cells (DCs) were exposed to Y. pestis grown at 26°C or 37°C, to their corresponding lipopolysaccharides (LPS-26°C or LPS-37°C), and to ligands of different Toll-like receptors (TLRs), such as LPS from Escherichia coli (TLR4), lipoprotein (TLR2), polyinosinic-polycytidylic acid (poly-IC) (TLR9), and their combinations. Production of cytokines was measured, along with expression of surface markers of DC maturation. Results: Y. pestis grown at 37°C or LPS-37°C induced much lower production of cytokines (such as tumor necrosis factor α and interleukins 1β, 10, and 12) by DCs than did Y. pestis grown at 26°C or LPS-26°C. Expression of the surface markers HLA-DR, CD86, and CD40 by DCs was also reduced in response to treatment with LPS-37°C compared with LPS-26°C. Pretreatment of DCs with LPS-37°C inhibited subsequent stimulation with LPS-26°C, control LPS from E. coli, lipoprotein, or poly-IC. Conclusions: LPS-37°C can inhibit stimulation of DCs not only via TLR4 signaling but also via TLR2 and TLR9.

Original languageEnglish (US)
Pages (from-to)1694-1702
Number of pages9
JournalJournal of Infectious Diseases
Volume200
Issue number11
DOIs
StatePublished - Nov 2009

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Yersinia pestis
Toll-Like Receptors
Dendritic Cells
Lipopolysaccharides
Poly I-C
Lipoproteins
Monocytes
Cytokines
Escherichia coli
Lipid A
Temperature
Plague
Acylation
HLA-DR Antigens
Interleukin-12
Interleukin-1
Interleukin-10
Tumor Necrosis Factor-alpha
Ligands
Bacteria

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Tetraacylated lipopolysaccharide of yersinia pestis can inhibit multiple toll-like receptor-mediated signaling pathways in human dendritic cells. / Telepnev, Maxim V.; Klimpel, Gary R.; Haithcoat, Judith; Knirel, Yuriy A.; Anisimov, Andrey P.; Motin, Vladimir.

In: Journal of Infectious Diseases, Vol. 200, No. 11, 11.2009, p. 1694-1702.

Research output: Contribution to journalArticle

Telepnev, Maxim V. ; Klimpel, Gary R. ; Haithcoat, Judith ; Knirel, Yuriy A. ; Anisimov, Andrey P. ; Motin, Vladimir. / Tetraacylated lipopolysaccharide of yersinia pestis can inhibit multiple toll-like receptor-mediated signaling pathways in human dendritic cells. In: Journal of Infectious Diseases. 2009 ; Vol. 200, No. 11. pp. 1694-1702.
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abstract = "Background: Yersinia pestis, the causative agent of plague, showed a temperature-dependent change in lipid A composition, with a reduced degree of acylation when bacteria were grown at 37°C (tetraacylated) versus ambient temperature (hexaacylated). Methods: Human monocytes and monocyte-derived dendritic cells (DCs) were exposed to Y. pestis grown at 26°C or 37°C, to their corresponding lipopolysaccharides (LPS-26°C or LPS-37°C), and to ligands of different Toll-like receptors (TLRs), such as LPS from Escherichia coli (TLR4), lipoprotein (TLR2), polyinosinic-polycytidylic acid (poly-IC) (TLR9), and their combinations. Production of cytokines was measured, along with expression of surface markers of DC maturation. Results: Y. pestis grown at 37°C or LPS-37°C induced much lower production of cytokines (such as tumor necrosis factor α and interleukins 1β, 10, and 12) by DCs than did Y. pestis grown at 26°C or LPS-26°C. Expression of the surface markers HLA-DR, CD86, and CD40 by DCs was also reduced in response to treatment with LPS-37°C compared with LPS-26°C. Pretreatment of DCs with LPS-37°C inhibited subsequent stimulation with LPS-26°C, control LPS from E. coli, lipoprotein, or poly-IC. Conclusions: LPS-37°C can inhibit stimulation of DCs not only via TLR4 signaling but also via TLR2 and TLR9.",
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