Tetracycline repressor allostery does not depend on divalent metal recognition

Sebastiaan Werten, Daniela Dalm, Gottfried Julius Palm, Christopher Cornelius Grimm, Winfried Hinrichs

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Genes that render bacteria resistant to tetracycline-derived antibiotics are tightly regulated by repressors of the TetR family. In their physiologically relevant, magnesium-complexed form, tetracyclines induce allosteric rearrangements in the TetR homodimer, leading to its release from the promoter and derepression of transcription. According to earlier crystallographic work, recognition of the tetracycline-associated magnesium ion by TetR is crucial and triggers the allosteric cascade. Nevertheless, the derivative 5a,6-anhydrotetracycline, which shows an increased affinity for TetR, causes promoter release even in the absence of magnesium. To resolve this paradox, it has been proposed that metal-free 5a,6-anhydrotetracycline acts via an exceptional, conformationally different induction mode that circumvents the normal magnesium requirement. We have tested this hypothesis by determining crystal structures of TetR-5a,6-anhydrotetracycline complexes in the presence of magnesium, ethylenediaminetetraacetic acid, or high concentrations of potassium. Analysis of these three structures reveals that, irrespective of the metal, the effects of 5a,6-anhydrotetracycline binding are indistinguishable from those of canonical induction by other tetracyclines. Together with a close scrutiny of the earlier evidence of a metal-triggered mechanism, these results demonstrate that magnesium recognition per se is not a prerequisite for tetracycline repressor allostery.

Original languageEnglish (US)
Pages (from-to)7990-7998
Number of pages9
JournalBiochemistry
Volume53
Issue number50
DOIs
StatePublished - Dec 23 2014

ASJC Scopus subject areas

  • Biochemistry

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    Werten, S., Dalm, D., Palm, G. J., Grimm, C. C., & Hinrichs, W. (2014). Tetracycline repressor allostery does not depend on divalent metal recognition. Biochemistry, 53(50), 7990-7998. https://doi.org/10.1021/bi5012805