TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs

Li Peng, Yong Zhou, Liang Dong, Rui Qi Chen, Guo Ying Sun, Tian Liu, Wen Zhuo Ran, Xiang Fang, Jian Xin Jiang, Cha Xiang Guan

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Triggering receptor expressed on myeloid cells 1 (TREM-1) increases the expression of TGF-β family genes, which are known as profibrogenic cytokines in the pathogenesis of pulmonary fibrosis. In this study, we determined whether TGF-β1 regulated the expression of TREM-1 in a mouse model of pulmonary fibrosis. The expression of TGF-β1 and TREM-1 was increased on day 7, 14, and 21 after single intratracheal injection of bleomycin (BLM). And there was positive correlation between the expression of TGF-β1 and TREM-1. TGF-β1 increased expression of TREM-1 mRNA and protein in a time- and dose-dependent manner in mouse macrophages. The expression of the activator protein 1 (AP-1) was increased in lung tissues from mouse after BLM injection and in mouse macrophages after TGF-β1 treatment, respectively. TGF-β1 significantly increased the relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter of TREM-1. But TGF-β1 had no effect on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutations in the AP-1 promoter binding site. In conclusion, we found the expression of TREM-1 was increased in lung tissues from mice with pulmonary fibrosis. TGF-β1 increased the expression of TREM-1 in mouse macrophages partly via the transcription factor AP-1.

Original languageEnglish (US)
Article number18946
JournalScientific reports
Volume6
DOIs
StatePublished - Jan 7 2016

ASJC Scopus subject areas

  • General

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