TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs

Li Peng, Yong Zhou, Liang Dong, Rui Qi Chen, Guo Ying Sun, Tian Liu, Wen Zhuo Ran, Xiang Fang, Jian Xin Jiang, Cha Xiang Guan

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Abstract

Triggering receptor expressed on myeloid cells 1 (TREM-1) increases the expression of TGF-β family genes, which are known as profibrogenic cytokines in the pathogenesis of pulmonary fibrosis. In this study, we determined whether TGF-β1 regulated the expression of TREM-1 in a mouse model of pulmonary fibrosis. The expression of TGF-β1 and TREM-1 was increased on day 7, 14, and 21 after single intratracheal injection of bleomycin (BLM). And there was positive correlation between the expression of TGF-β1 and TREM-1. TGF-β1 increased expression of TREM-1 mRNA and protein in a time- and dose-dependent manner in mouse macrophages. The expression of the activator protein 1 (AP-1) was increased in lung tissues from mouse after BLM injection and in mouse macrophages after TGF-β1 treatment, respectively. TGF-β1 significantly increased the relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter of TREM-1. But TGF-β1 had no effect on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutations in the AP-1 promoter binding site. In conclusion, we found the expression of TREM-1 was increased in lung tissues from mice with pulmonary fibrosis. TGF-β1 increased the expression of TREM-1 in mouse macrophages partly via the transcription factor AP-1.

Original languageEnglish (US)
Article number18946
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 7 2016

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Myeloid Cells
Up-Regulation
Lung
Pulmonary Fibrosis
Transcription Factor AP-1
Macrophages
Luciferases
Plasmids
Injections
Bleomycin
Transcription Factors
Binding Sites
Cytokines
Messenger RNA
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Peng, L., Zhou, Y., Dong, L., Chen, R. Q., Sun, G. Y., Liu, T., ... Guan, C. X. (2016). TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs. Scientific Reports, 6, [18946]. https://doi.org/10.1038/srep18946

TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs. / Peng, Li; Zhou, Yong; Dong, Liang; Chen, Rui Qi; Sun, Guo Ying; Liu, Tian; Ran, Wen Zhuo; Fang, Xiang; Jiang, Jian Xin; Guan, Cha Xiang.

In: Scientific Reports, Vol. 6, 18946, 07.01.2016.

Research output: Contribution to journalArticle

Peng, L, Zhou, Y, Dong, L, Chen, RQ, Sun, GY, Liu, T, Ran, WZ, Fang, X, Jiang, JX & Guan, CX 2016, 'TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs', Scientific Reports, vol. 6, 18946. https://doi.org/10.1038/srep18946
Peng, Li ; Zhou, Yong ; Dong, Liang ; Chen, Rui Qi ; Sun, Guo Ying ; Liu, Tian ; Ran, Wen Zhuo ; Fang, Xiang ; Jiang, Jian Xin ; Guan, Cha Xiang. / TGF-β1 upregulates the expression of triggering receptor expressed on myeloid cells 1 in murine lungs. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Triggering receptor expressed on myeloid cells 1 (TREM-1) increases the expression of TGF-β family genes, which are known as profibrogenic cytokines in the pathogenesis of pulmonary fibrosis. In this study, we determined whether TGF-β1 regulated the expression of TREM-1 in a mouse model of pulmonary fibrosis. The expression of TGF-β1 and TREM-1 was increased on day 7, 14, and 21 after single intratracheal injection of bleomycin (BLM). And there was positive correlation between the expression of TGF-β1 and TREM-1. TGF-β1 increased expression of TREM-1 mRNA and protein in a time- and dose-dependent manner in mouse macrophages. The expression of the activator protein 1 (AP-1) was increased in lung tissues from mouse after BLM injection and in mouse macrophages after TGF-β1 treatment, respectively. TGF-β1 significantly increased the relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter of TREM-1. But TGF-β1 had no effect on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutations in the AP-1 promoter binding site. In conclusion, we found the expression of TREM-1 was increased in lung tissues from mice with pulmonary fibrosis. TGF-β1 increased the expression of TREM-1 in mouse macrophages partly via the transcription factor AP-1.",
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