The 120 kDa outer membrane protein of ehrlichia chaffeensis

Preferential expression on dense-core cells and gene expression in Escherichia coli associated with attachment and entry

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Abstract

The immunodominant 120 kDa protein (p120) of Ehrlichia chaffeensis was demonstrated to be exposed on the surface of purified whole ehrlichial cells examined by immunoelectron microscopy with a rabbit antibody against a portion of the domain containing tandem repeat units. In the intracellular location, the 120 kDa protein was detected by immunoelectron microscopy in the outer membrane of the cell wall of dense-core forms of the ehrlichiae in infected canine macrophage-like cells and as a component of the intramorular fibrillary matrix. No 120 kDa protein was detected in the cell wall of ehrlichial reticulate cells. Recombinant Escherichia coli with a plasmid containing the entire 120 kDa protein gene, but no bacteria with non-recombinant plasmid, attached to the surface of HeLa cells as visualized by electron microscopy. Some of the recombinant 120 kDa protein expressing E. coli invaded the HeLa cells as determined by gentamicin protection assays and by intravacuolar localization ultrastructurally. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalMicrobial Pathogenesis
Volume28
Issue number2
DOIs
StatePublished - 2000

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Ehrlichia chaffeensis
Membrane Proteins
Immunoelectron Microscopy
Escherichia coli
HeLa Cells
Gene Expression
Cell Wall
Plasmids
Ehrlichia
Proteins
Tandem Repeat Sequences
Escherichia coli Proteins
Gentamicins
Canidae
Electron Microscopy
Macrophages
Rabbits
Bacteria
Membranes
Antibodies

Keywords

  • Adhesin
  • Ehrlichia chaffeensis
  • Immunoelectron microscopy
  • Invasion
  • Outer membrane protein
  • Recombinant protein

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

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title = "The 120 kDa outer membrane protein of ehrlichia chaffeensis: Preferential expression on dense-core cells and gene expression in Escherichia coli associated with attachment and entry",
abstract = "The immunodominant 120 kDa protein (p120) of Ehrlichia chaffeensis was demonstrated to be exposed on the surface of purified whole ehrlichial cells examined by immunoelectron microscopy with a rabbit antibody against a portion of the domain containing tandem repeat units. In the intracellular location, the 120 kDa protein was detected by immunoelectron microscopy in the outer membrane of the cell wall of dense-core forms of the ehrlichiae in infected canine macrophage-like cells and as a component of the intramorular fibrillary matrix. No 120 kDa protein was detected in the cell wall of ehrlichial reticulate cells. Recombinant Escherichia coli with a plasmid containing the entire 120 kDa protein gene, but no bacteria with non-recombinant plasmid, attached to the surface of HeLa cells as visualized by electron microscopy. Some of the recombinant 120 kDa protein expressing E. coli invaded the HeLa cells as determined by gentamicin protection assays and by intravacuolar localization ultrastructurally. (C) 2000 Academic Press.",
keywords = "Adhesin, Ehrlichia chaffeensis, Immunoelectron microscopy, Invasion, Outer membrane protein, Recombinant protein",
author = "Vsevolod Popov and Yu, {Xue Jie} and David Walker",
year = "2000",
doi = "10.1006/mpat.1999.0327",
language = "English (US)",
volume = "28",
pages = "71--80",
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T1 - The 120 kDa outer membrane protein of ehrlichia chaffeensis

T2 - Preferential expression on dense-core cells and gene expression in Escherichia coli associated with attachment and entry

AU - Popov, Vsevolod

AU - Yu, Xue Jie

AU - Walker, David

PY - 2000

Y1 - 2000

N2 - The immunodominant 120 kDa protein (p120) of Ehrlichia chaffeensis was demonstrated to be exposed on the surface of purified whole ehrlichial cells examined by immunoelectron microscopy with a rabbit antibody against a portion of the domain containing tandem repeat units. In the intracellular location, the 120 kDa protein was detected by immunoelectron microscopy in the outer membrane of the cell wall of dense-core forms of the ehrlichiae in infected canine macrophage-like cells and as a component of the intramorular fibrillary matrix. No 120 kDa protein was detected in the cell wall of ehrlichial reticulate cells. Recombinant Escherichia coli with a plasmid containing the entire 120 kDa protein gene, but no bacteria with non-recombinant plasmid, attached to the surface of HeLa cells as visualized by electron microscopy. Some of the recombinant 120 kDa protein expressing E. coli invaded the HeLa cells as determined by gentamicin protection assays and by intravacuolar localization ultrastructurally. (C) 2000 Academic Press.

AB - The immunodominant 120 kDa protein (p120) of Ehrlichia chaffeensis was demonstrated to be exposed on the surface of purified whole ehrlichial cells examined by immunoelectron microscopy with a rabbit antibody against a portion of the domain containing tandem repeat units. In the intracellular location, the 120 kDa protein was detected by immunoelectron microscopy in the outer membrane of the cell wall of dense-core forms of the ehrlichiae in infected canine macrophage-like cells and as a component of the intramorular fibrillary matrix. No 120 kDa protein was detected in the cell wall of ehrlichial reticulate cells. Recombinant Escherichia coli with a plasmid containing the entire 120 kDa protein gene, but no bacteria with non-recombinant plasmid, attached to the surface of HeLa cells as visualized by electron microscopy. Some of the recombinant 120 kDa protein expressing E. coli invaded the HeLa cells as determined by gentamicin protection assays and by intravacuolar localization ultrastructurally. (C) 2000 Academic Press.

KW - Adhesin

KW - Ehrlichia chaffeensis

KW - Immunoelectron microscopy

KW - Invasion

KW - Outer membrane protein

KW - Recombinant protein

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U2 - 10.1006/mpat.1999.0327

DO - 10.1006/mpat.1999.0327

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EP - 80

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

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