The 1996 Moyer Award

Effects of endotoxin on the Th1/Th2 response in humans

S. Zimmer, V. Pollard, G. D. Marshall, Roberto Garofalo, D. Traber, Donald Prough, David Herndon

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Monocyte/T-cell interactions play a critical role in the systemic response to infection. Distinct patterns of cytokines are produced by two different types of T-helper cells (Th). Th1 cells secrete interleukin-2 (IL- 2) and interferon-γ (IFN-γ), whereas Th2 cells produce IL-4, IL-5, IL-6, IL-10, and IL-13. In volunteers systemic endotoxin administration initiates many features of gram-negative sepsis including cytokine release, but the patterns (i.e., Th1/Th2 patterns) have not yet been studied. In this institutional review board-approved study we investigated the effect of an intravenous bolus of endotoxin from Escherichia coli (4 ng/kg body weight) on the Th1/Th2 response in four female and four male volunteers (mean age 27.1 ± 0.8 years). Plasma cytokine levels for IL-2, IL-4, IL-10, IL-12, and IFN- γ and heart rate, mean arterial pressure, temperature, white blood cell, and differential blood count were determined before and hourly for 5 hours after endotoxin administration. All volunteers had tachycardia, decreased mean arterial pressure, fever, and leukocytosis. IL-10 was significantly (p < 0.05) elevated (9.4 ± 3.9 pg/ml vs 60.9 ± 19.3 pg/ml) 3 hours after endotoxin was administered, whereas IL-2 levels were decreased (69 ± 26 U/ml vs 30.6 ± 14.9 U/ml). IL-4 and IFN-γ were not detectable in plasma. No changes were seen in the plasma levels of IL-12. Systemic responses did not correlate with changes in cytokine levels. Cytokine patterns found in this study suggest that after low-dose endotoxin administration the T-cell immune response is shifted towards the Th2 cell type response. This early shift towards a Th2 cell response may contribute to the depressed cell-mediated immune response associated with sepsis.

Original languageEnglish (US)
Pages (from-to)491-496
Number of pages6
JournalJournal of Burn Care and Rehabilitation
Volume17
Issue number6
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

Endotoxins
Th2 Cells
Cytokines
Interleukin-4
Interleukin-10
Interferons
Interleukin-2
Volunteers
Interleukin-12
Sepsis
Arterial Pressure
T-Lymphocytes
Th1 Cells
Interleukin-13
Research Ethics Committees
Interleukin-5
Leukocytosis
Helper-Inducer T-Lymphocytes
Tachycardia
Cell Communication

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Zimmer, S., Pollard, V., Marshall, G. D., Garofalo, R., Traber, D., Prough, D., & Herndon, D. (1996). The 1996 Moyer Award: Effects of endotoxin on the Th1/Th2 response in humans. Journal of Burn Care and Rehabilitation, 17(6), 491-496.

The 1996 Moyer Award : Effects of endotoxin on the Th1/Th2 response in humans. / Zimmer, S.; Pollard, V.; Marshall, G. D.; Garofalo, Roberto; Traber, D.; Prough, Donald; Herndon, David.

In: Journal of Burn Care and Rehabilitation, Vol. 17, No. 6, 11.1996, p. 491-496.

Research output: Contribution to journalArticle

Zimmer, S, Pollard, V, Marshall, GD, Garofalo, R, Traber, D, Prough, D & Herndon, D 1996, 'The 1996 Moyer Award: Effects of endotoxin on the Th1/Th2 response in humans', Journal of Burn Care and Rehabilitation, vol. 17, no. 6, pp. 491-496.
Zimmer, S. ; Pollard, V. ; Marshall, G. D. ; Garofalo, Roberto ; Traber, D. ; Prough, Donald ; Herndon, David. / The 1996 Moyer Award : Effects of endotoxin on the Th1/Th2 response in humans. In: Journal of Burn Care and Rehabilitation. 1996 ; Vol. 17, No. 6. pp. 491-496.
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